Chenopodium quinoa willd EST-SSR molecular marker, development method of chenopodium quinoa willd EST-SSR molecular marker and application of chenopodium quinoa willd EST-SSR molecular marker

The invention relates to the technical field of molecular markers, in particular to a chenopodium quinoa willd EST-SSR (Expressed Sequence Tag-Simple Sequence Repeat) molecular marker, a development method of the chenopodium quinoa willd EST-SSR molecular marker and application of the chenopodium quinoa willd EST-SSR molecular marker. A group of chenopodium quinoa willd EST-SSR molecular markers comprises forward primers and reverse primers corresponding to 66 sites. The development method of the chenopodium quinoa willd EST-SSR molecular marker comprises the following steps of: 1, obtaining RNA-seq and EST sequences from an NCBI (National Center of Biotechnology Information) database; 2, using bioinformatics software to process, assemble and splice sequences into unigenes, and performing SSR site searching on the unigenes to obtain the EST-SSR; and 3, designing EST-SSR primer amplification chenopodium quinoa willd genome DNA for verifying the EST-SSR, and using the verified EST-SSR molecular marker to realize application of genetic diversity analysis in chenopodiaceae germplasm. The EST-SSR molecular marker developed on the basis of a public database has the advantages of high efficiency, high success rate, rich polymorphism and high universality.