Method for directly immobilizing tannase in fermentation liquor
The invention discloses a method for directly immobilizing tannase in fermentation liquor. The method includes (1), fermenting Tie Guanyin tea stems by the aid of aspergillus tubingensis to obtain free crude tannase; (2), utilizing hydrophilic super-paramagnetic nano-particles as carriers for immobi...
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| creator | XIAO ANFENG CAI HUINONG YANG QIUMING DU XIPING LI LIJUN NI HUI HUANG GAOLING WU CHANGZHENG CHEN YANHONG |
| description | The invention discloses a method for directly immobilizing tannase in fermentation liquor. The method includes (1), fermenting Tie Guanyin tea stems by the aid of aspergillus tubingensis to obtain free crude tannase; (2), utilizing hydrophilic super-paramagnetic nano-particles as carriers for immobilizing free tannase; (3), immobilizing the free tannase on the nano-particles by a direct immobilizing process, a glutaraldehyde cross-linking process and a carbodiimide covalent binding process so as to obtain liquid immobilized tannase; (4), carrying out vacuum freeze-drying on the liquid immobilized tannase to obtain powder immobilized tannase. The aspergillus tubingensis is used as an enzyme producing strain. The method has the advantages that the magnetic nano immobilized tannase is directly immobilized in the fermentation crude enzyme liquor and accordingly can be easily and quickly prepared; the immobilized tannase is excellent in enzyme activity, storage stability, reusability and magnetic control performance. |
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The method includes (1), fermenting Tie Guanyin tea stems by the aid of aspergillus tubingensis to obtain free crude tannase; (2), utilizing hydrophilic super-paramagnetic nano-particles as carriers for immobilizing free tannase; (3), immobilizing the free tannase on the nano-particles by a direct immobilizing process, a glutaraldehyde cross-linking process and a carbodiimide covalent binding process so as to obtain liquid immobilized tannase; (4), carrying out vacuum freeze-drying on the liquid immobilized tannase to obtain powder immobilized tannase. The aspergillus tubingensis is used as an enzyme producing strain. 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The method includes (1), fermenting Tie Guanyin tea stems by the aid of aspergillus tubingensis to obtain free crude tannase; (2), utilizing hydrophilic super-paramagnetic nano-particles as carriers for immobilizing free tannase; (3), immobilizing the free tannase on the nano-particles by a direct immobilizing process, a glutaraldehyde cross-linking process and a carbodiimide covalent binding process so as to obtain liquid immobilized tannase; (4), carrying out vacuum freeze-drying on the liquid immobilized tannase to obtain powder immobilized tannase. The aspergillus tubingensis is used as an enzyme producing strain. 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The method includes (1), fermenting Tie Guanyin tea stems by the aid of aspergillus tubingensis to obtain free crude tannase; (2), utilizing hydrophilic super-paramagnetic nano-particles as carriers for immobilizing free tannase; (3), immobilizing the free tannase on the nano-particles by a direct immobilizing process, a glutaraldehyde cross-linking process and a carbodiimide covalent binding process so as to obtain liquid immobilized tannase; (4), carrying out vacuum freeze-drying on the liquid immobilized tannase to obtain powder immobilized tannase. The aspergillus tubingensis is used as an enzyme producing strain. The method has the advantages that the magnetic nano immobilized tannase is directly immobilized in the fermentation crude enzyme liquor and accordingly can be easily and quickly prepared; the immobilized tannase is excellent in enzyme activity, storage stability, reusability and magnetic control performance.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for directly immobilizing tannase in fermentation liquor |
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