Method for identifying non-developing substances on thin layer plate by using surface-enhanced Raman spectrum

The invention provides a method for identifying non-developing substances on a thin layer plate by using a surface-enhanced Raman spectrum. After substances to be detected are separated by the thin layer plate, a Raman spectrograph is used for scanning the thin layer plate. The method is characterized in that a vertical mark from a sample application spot on the thin layer plate to the front edge of a solvent is marked as a detection line, and a surface enhancing agent is dropwise added onto the sample application spot; the Raman spectrograph is used for scanning point by point along the detection line from the sample application spot and the scanning is performed for one time every m millimeters and is stopped after n times; the surface enhancing agent is dropwise added once at a scanning stopping part; after the dripping is finished, the Raman spectrograph is used for continually scanning for one time every m millimeters, and the scanning is suspended after n times; after the surface enhancing agent is dropwise added, the Raman spectrograph is used for continually scanning; the operation is repeated until the front edge of the solvent is scanned to obtain a series of Raman spectrum charts; and the obtained Raman spectrum charts are compared with standard substance charts to finish the identification of the substances to be detected. According to the technical scheme, the non-developing substances on the thin layer plate can be identified, the accuracy is good and the applicable range is wide.