种从白柳皮提取水杨苷的方法

本发明涉及从白柳皮白柳皮中提取水杨苷的方法，采用灭菌、浸润、酶解提取、分离、干燥的工艺，得到了富含水杨苷的产品。本发明创造性地采用了酶解前灭酶和食盐水浸泡的工艺，并通过系统筛选确定酶解反应的酶种类，并经过两次柱层析分离，微波干燥后，得到水杨苷含量超过95%的白柳皮提取物。整个方法无需使用生石灰，且所用材料均为常规材料，成本低且收率高、且溶剂可循环利用，能降低环境污染，易于产业化。 The invention relates to a method for extracting salicin from white willow bark. According to the method, a salicin-rich product is obtained through a process including sterilization, infiltration, enzymolysis extraction, separation and drying. The method creatively adopts the process in which enzyme deactivation and salt solution soaking are performed before enzymolysis, the kind of enzyme in enzymolysis reaction is determined by system screening, and a white willow bark extract containing more than 95 percent of salicin is obtained through twice column chromatography separation and microwave drying. Quicklime is not needed to be used in the whole method, all materials used in the method are conventional materials, the cost is low, the yield is high, a solvent can be used repeatedly, environmental pollution is reduced,