Method for determining nitroimidazole drug in bee pollen powder through combination of rapid solvent extraction-gel chromatography purification-LC/MS/MS

The present invention relates to a detection method for a nitroimidazole drug in bee pollen powder, particularly to a method for determining a nitroimidazole drug in bee pollen powder through combination of rapid solvent extraction-gel chromatography purification-LC/MS/MS. According to the method, ethyl acetate is adopted as an extraction solvent, extraction with an accelerated solvent extractor (ASE) and gel chromatography purification (GPC) are performed, a liquid chromatography-mass spectrometry multiple reaction positive ion monitoring mode is used to determine, a matrix-matched standard curve isotope internal standard method is used to quantitate, and the results comprise the nitroimidazole compound detection limit is 1.5 [mu]g.kg, and the quantification limit is 5.0 [mu]g.kg. According to the present invention, the method recovery rate is 93.5-114.2%, and the relative standard deviation is 0.3-5.2%; and the conclusion is that the method has advantages of high automation degree, high sensitivity, accurate qualitative and quantitative result, and the like.