Application method of astragalus polysaccharide in antagonism of dairy cow mammary epithelial cells apoptosis

The invention discloses an application method of astragalus polysaccharide in antagonism of dairy cow mammary epithelial cells apoptosis. The method comprises the following steps: (1) obtaining 2-3 generations of dairy cow mammary epithelial cells; (2) grouping and processing cells; (3) detecting cell survival rate employing a CCK-8 method; (4) detecting a cell cycle employing flow cytometry; (5) detecting the change condition of nucleic acid of apoptotic cells employing a DNA ladder; (6) observing the internal morphological structure change of the cells through a transmission electron microscope; (7) detecting inflammation-associated cytokines content in a cell supernatant employing radioimmunoassay; and (8) detecting inflammation-related enzyme activity in the supernatant employing a biochemical kit method. Through the method disclosed by the invention, the action of the astragalus polysaccharide for antagonism of dairy cow mammary epithelial cells apoptosis is discussed; and the method is efficient, simple, convenient, feasible, and accurate in result.