Preparation and application of anti-vibrio parahaemolyticus OMPK (outer membrane protein k) egg yolk antibody
The invention discloses a preparation method for an anti-vibrio parahaemolyticus ompk (outer membrane protein k) egg yolk antibody, and belongs to the technical field of biological product preparation. The preparation method comprises the following steps: performing inoculation immunization on a lay...
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| creator | WANG XUEPENG YAN MAOCANG HAO JINTING ZHANG SAILE HU WEILI |
| description | The invention discloses a preparation method for an anti-vibrio parahaemolyticus ompk (outer membrane protein k) egg yolk antibody, and belongs to the technical field of biological product preparation. The preparation method comprises the following steps: performing inoculation immunization on a laying hen by utilizing vibrio parahaemolyticus ompk, removing egg white of an egg produced by the inoculated hen, rinsing the egg with distilled water to obtain pure egg yolk liquid, performing centrifuging on the pure egg yolk liquid, collecting supernatant liquid, preparing a crude egg yolk antibody with a saturated ammonium sulfate solution precipitation method, further performing purification by applying a gel chromatographic column, and obtaining the purified anti-vibrio parahaemolyticus ompk egg yolk antibody. A quick detection method for detecting vibrio parahaemolyticus by the purified anti-vibrio parahaemolyticus OMPK egg yolk antibody has relatively high specificity. |
| format | Patent |
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The preparation method comprises the following steps: performing inoculation immunization on a laying hen by utilizing vibrio parahaemolyticus ompk, removing egg white of an egg produced by the inoculated hen, rinsing the egg with distilled water to obtain pure egg yolk liquid, performing centrifuging on the pure egg yolk liquid, collecting supernatant liquid, preparing a crude egg yolk antibody with a saturated ammonium sulfate solution precipitation method, further performing purification by applying a gel chromatographic column, and obtaining the purified anti-vibrio parahaemolyticus ompk egg yolk antibody. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Preparation and application of anti-vibrio parahaemolyticus OMPK (outer membrane protein k) egg yolk antibody |
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