Applications of calcium carboxylic acid and derivatives thereof in phosphorylated protein fluorescence detection

The present invention relates to a phosphorylated protein fluorescence detection technology, specifically to applications of calcium carboxylic acid and derivatives thereof in phosphorylated protein fluorescence detection. The present invention further provides a method for adopting calcium carboxyl...

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Hauptverfasser: HONG GUOYING, ZHOU AYI, LIU ZHIGUO, CONG WEITAO, ZHU ZHONGXIN, SHEN JIAYI, ZHU XINLIANG, JIN LITAI
Format: Patent
Sprache:eng
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Zusammenfassung:The present invention relates to a phosphorylated protein fluorescence detection technology, specifically to applications of calcium carboxylic acid and derivatives thereof in phosphorylated protein fluorescence detection. The present invention further provides a method for adopting calcium carboxylic acid to carry out phosphorylated protein fluorescence detection, wherein the method comprises dyeing after protein sample electrophoresis, double-step decolorization, and detection. According to the invention, advantages of good specificity, low detection limit, simple operation, stable reproducibility, good dyeing effect, good linearity, good reversibility, good compatibility, safe and environmental protection use, low cost and the like are provided.