Qualitative PCR (polymerase chain reaction) detection primers and detection method for specificity of transgenic alfalfa J101 strain
The invention discloses qualitative PCR (polymerase chain reaction) detection primers and a detection method for specificity of a transgenic alfalfa J101 strain. A pair of specific primers is designed and provided for the first time and a qualitative PCR detection system is successfully established...
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| creator | LYU YINGZI WANG DINGGUO YAO BAIHUI JIANG XIANG LIN HUIJIAO ZHENG GAOBIN LIU ERLONG TANG JIE QIN ZHUOMIN FAN WUJIANG LU LI LIN XUEQIN |
| description | The invention discloses qualitative PCR (polymerase chain reaction) detection primers and a detection method for specificity of a transgenic alfalfa J101 strain. A pair of specific primers is designed and provided for the first time and a qualitative PCR detection system is successfully established according to an adjacent area between a 5'-end exogenous insert fragment P-eFMV of a genome DNA sequence of the transgenic alfalfa strain J101 which is not issued with an agricultural transgene bio-safety certificate by the agriculture department of China and an alfalfa genome DNA. Results show that the qualitative PCR detection method disclosed by the invention has good specificity; the detected lowest DNA concentration is 80pg, which is equivalent to genome DNA of 50 copies; and the detection method is suitable for quick and stable qualitative detection of the J101 strain. |
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A pair of specific primers is designed and provided for the first time and a qualitative PCR detection system is successfully established according to an adjacent area between a 5'-end exogenous insert fragment P-eFMV of a genome DNA sequence of the transgenic alfalfa strain J101 which is not issued with an agricultural transgene bio-safety certificate by the agriculture department of China and an alfalfa genome DNA. Results show that the qualitative PCR detection method disclosed by the invention has good specificity; the detected lowest DNA concentration is 80pg, which is equivalent to genome DNA of 50 copies; and the detection method is suitable for quick and stable qualitative detection of the J101 strain.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2015</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20150225&DB=EPODOC&CC=CN&NR=104372088A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76419</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20150225&DB=EPODOC&CC=CN&NR=104372088A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LYU YINGZI</creatorcontrib><creatorcontrib>WANG DINGGUO</creatorcontrib><creatorcontrib>YAO BAIHUI</creatorcontrib><creatorcontrib>JIANG XIANG</creatorcontrib><creatorcontrib>LIN HUIJIAO</creatorcontrib><creatorcontrib>ZHENG GAOBIN</creatorcontrib><creatorcontrib>LIU ERLONG</creatorcontrib><creatorcontrib>TANG JIE</creatorcontrib><creatorcontrib>QIN ZHUOMIN</creatorcontrib><creatorcontrib>FAN WUJIANG</creatorcontrib><creatorcontrib>LU LI</creatorcontrib><creatorcontrib>LIN XUEQIN</creatorcontrib><title>Qualitative PCR (polymerase chain reaction) detection primers and detection method for specificity of transgenic alfalfa J101 strain</title><description>The invention discloses qualitative PCR (polymerase chain reaction) detection primers and a detection method for specificity of a transgenic alfalfa J101 strain. A pair of specific primers is designed and provided for the first time and a qualitative PCR detection system is successfully established according to an adjacent area between a 5'-end exogenous insert fragment P-eFMV of a genome DNA sequence of the transgenic alfalfa strain J101 which is not issued with an agricultural transgene bio-safety certificate by the agriculture department of China and an alfalfa genome DNA. 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A pair of specific primers is designed and provided for the first time and a qualitative PCR detection system is successfully established according to an adjacent area between a 5'-end exogenous insert fragment P-eFMV of a genome DNA sequence of the transgenic alfalfa strain J101 which is not issued with an agricultural transgene bio-safety certificate by the agriculture department of China and an alfalfa genome DNA. Results show that the qualitative PCR detection method disclosed by the invention has good specificity; the detected lowest DNA concentration is 80pg, which is equivalent to genome DNA of 50 copies; and the detection method is suitable for quick and stable qualitative detection of the J101 strain.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Qualitative PCR (polymerase chain reaction) detection primers and detection method for specificity of transgenic alfalfa J101 strain |
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