Primer and method for measuring titer of haiophilic archaea phage SNJ1

The invention provides a primer and a method for measuring the titer of a haiophilic archaea phage SNJ1. The primer comprises an upstream primer 5'-ACGAGTGGCGGCAGGTTT-3' and a downstream primer 5'-TCCCGATGATGCGGTTGT-3'; according to the method for measuring the titer of the haiop...

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Bibliographische Detailangaben
Hauptverfasser:	MEI YUNJUN, HUANG YONGCHI, HE CONGCONG
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a primer and a method for measuring the titer of a haiophilic archaea phage SNJ1. The primer comprises an upstream primer 5'-ACGAGTGGCGGCAGGTTT-3' and a downstream primer 5'-TCCCGATGATGCGGTTGT-3'; according to the method for measuring the titer of the haiophilic archaea phage SNJ1 by use of the primer, firstly, a phage preserving fluid to be tested is mixed with the upstream primer, the downstream primer, a quantitative PCR kit and sterile water to have a quantitative PCR reaction; after the reaction ends, the Ct value is measured; and finally, the titer of the haiophilic archaea phage SNJ1 is calculated according to the Ct value. The primer and the method for measuring the titer of the haiophilic archaea phage SNJ1 are simple and fast, and strong in specificity, high in accuracy and good in reproducibility.