LAMP primer of staphylococcus aureus and application method of LAMP primer

The invention discloses an LAMP primer of staphylococcus aureus. The LAMP primer comprises two inner primers (FIP and BIP) and two outer primers (F3 and B3), namely, F3 as shown in the sequence SEQ ID NO:1, B3 as shown in the sequence SEQ ID NO:2, FIP as shown in the sequence SEQ ID NO:3 and BIP as shown in the sequence SEQ ID NO:4. Aiming at the specific nuc gene of the staphylococcus aureus, a group of primers is designed, and a sensitive and specific staphylococcus aureus LAMP detection method is established by using the primers. The experiment shows that the detection sensitivity and the detection limit of the LAMP detection method are 100 times of those of a PCR method; whether green fluorescence is generated or not can be observed with naked eyes so as to confirm whether the reaction is performed, so that the result judgment can be simple, convenient and feasible; compared with a PCR technique, the method disclosed by the invention has the advantages that the time and the labor can be saved, no expensive instruments or tedious electrophoretic analysis is needed, and the application method disclosed by the invention is particularly applicable to use in base levels.