Rice vacuum permeation genetic transformation device

The invention relates to a rice vacuum permeation genetic transformation device. The middle part of a supporting table top of the device is provided with a through hole; a bottom plate is arranged on the supporting table; the middle parts of the upper and lower surfaces of the bottom plate are respe...

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Hauptverfasser: LUO HONGMEI, LI EXIAN, KE XUE, ZHONG QIAOFANG, XIAO SUQIN, CHEN YUE, HUANG XINGQI, CHEN LING, WANG BO, JIANG CHUNMIAO, LI WEIJIAO, FU JIAN, ZHANG DUNYU, CHENG ZAIQUAN, JIANG CONG, ZENG MIN, LI DINGQIN, YU TENGQIONG, YIN FUYOU, WANG LINGXIAN
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creator LUO HONGMEI
LI EXIAN
KE XUE
ZHONG QIAOFANG
XIAO SUQIN
CHEN YUE
HUANG XINGQI
CHEN LING
WANG BO
JIANG CHUNMIAO
LI WEIJIAO
FU JIAN
ZHANG DUNYU
CHENG ZAIQUAN
JIANG CONG
ZENG MIN
LI DINGQIN
YU TENGQIONG
YIN FUYOU
WANG LINGXIAN
description The invention relates to a rice vacuum permeation genetic transformation device. The middle part of a supporting table top of the device is provided with a through hole; a bottom plate is arranged on the supporting table; the middle parts of the upper and lower surfaces of the bottom plate are respectively provided with an upper cylinder and a lower cylinder; a magnet ring II and a magnet ring I are respectively sheathed on the upper cylinder and lower cylinder; the adjacent magnetic poles of the magnet ring I and magnet ring II are homopolar; the lower cylinder and magnet ring I penetrate through the through hole and are positioned under the table top; a vacuum permeation genetic transformation chamber is arranged on the bottom plate; the upper cylinder is positioned in the chamber; a bracket is arranged on the magnet ring II and provided with a permeation solution container; the upper inner wall of the chamber is provided with an annular loading platform, an air vent pipe with a valve, and a vacuum air exhaust connecting pipe; and the bottom end port and top end port of the chamber are respectively provided with a seal ring. The device can perform transformation on the plant to be transformed in the vacuum state immediately after the plant starts contacting the permeation solution, implements in-situ genetic transformation on the rice in the vacuum state in deed, and enhances the infecting potential of agrobacterium for the plant. The device is applicable to both smaller and bigger crop individuals.
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The middle part of a supporting table top of the device is provided with a through hole; a bottom plate is arranged on the supporting table; the middle parts of the upper and lower surfaces of the bottom plate are respectively provided with an upper cylinder and a lower cylinder; a magnet ring II and a magnet ring I are respectively sheathed on the upper cylinder and lower cylinder; the adjacent magnetic poles of the magnet ring I and magnet ring II are homopolar; the lower cylinder and magnet ring I penetrate through the through hole and are positioned under the table top; a vacuum permeation genetic transformation chamber is arranged on the bottom plate; the upper cylinder is positioned in the chamber; a bracket is arranged on the magnet ring II and provided with a permeation solution container; the upper inner wall of the chamber is provided with an annular loading platform, an air vent pipe with a valve, and a vacuum air exhaust connecting pipe; and the bottom end port and top end port of the chamber are respectively provided with a seal ring. The device can perform transformation on the plant to be transformed in the vacuum state immediately after the plant starts contacting the permeation solution, implements in-situ genetic transformation on the rice in the vacuum state in deed, and enhances the infecting potential of agrobacterium for the plant. 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The middle part of a supporting table top of the device is provided with a through hole; a bottom plate is arranged on the supporting table; the middle parts of the upper and lower surfaces of the bottom plate are respectively provided with an upper cylinder and a lower cylinder; a magnet ring II and a magnet ring I are respectively sheathed on the upper cylinder and lower cylinder; the adjacent magnetic poles of the magnet ring I and magnet ring II are homopolar; the lower cylinder and magnet ring I penetrate through the through hole and are positioned under the table top; a vacuum permeation genetic transformation chamber is arranged on the bottom plate; the upper cylinder is positioned in the chamber; a bracket is arranged on the magnet ring II and provided with a permeation solution container; the upper inner wall of the chamber is provided with an annular loading platform, an air vent pipe with a valve, and a vacuum air exhaust connecting pipe; and the bottom end port and top end port of the chamber are respectively provided with a seal ring. The device can perform transformation on the plant to be transformed in the vacuum state immediately after the plant starts contacting the permeation solution, implements in-situ genetic transformation on the rice in the vacuum state in deed, and enhances the infecting potential of agrobacterium for the plant. The device is applicable to both smaller and bigger crop individuals.</abstract><oa>free_for_read</oa></addata></record>
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subjects APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Rice vacuum permeation genetic transformation device
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