Split inteins and uses thereof
The present invention relates generally to robust split inteins. The split inteins described herein are active over a large temperature range, including temperatures as low as 0 DEG C, over a wide pH range, and in the presence of chaotropic salts. The split inteins also show high tolerance to sequen...
Gespeichert in:
Hauptverfasser: | , , , |
---|---|
Format: | Patent |
Sprache: | chi ; eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | |
---|---|
container_issue | |
container_start_page | |
container_title | |
container_volume | |
creator | MARCO FELIU DIDAC SCHMIDT STEFAN ROBERT CARVAJAL VALLEJOS PATRICIA KARINA PALLISSE BERGWERF ROSER |
description | The present invention relates generally to robust split inteins. The split inteins described herein are active over a large temperature range, including temperatures as low as 0 DEG C, over a wide pH range, and in the presence of chaotropic salts. The split inteins also show high tolerance to sequence variability in fused heterologous polypeptides and therefore are useful in protein purification and engineering techniques. |
format | Patent |
fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN104053779A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN104053779A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN104053779A3</originalsourceid><addsrcrecordid>eNrjZJALLsjJLFHIzCtJzcwrVkjMS1EoLU4tVijJSC1KzU_jYWBNS8wpTuWF0twMim6uIc4euqkF-fGpxQWJyal5qSXxzn6GBiYGpsbm5paOxsSoAQDxDiRz</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Split inteins and uses thereof</title><source>esp@cenet</source><creator>MARCO FELIU DIDAC ; SCHMIDT STEFAN ROBERT ; CARVAJAL VALLEJOS PATRICIA KARINA ; PALLISSE BERGWERF ROSER</creator><creatorcontrib>MARCO FELIU DIDAC ; SCHMIDT STEFAN ROBERT ; CARVAJAL VALLEJOS PATRICIA KARINA ; PALLISSE BERGWERF ROSER</creatorcontrib><description>The present invention relates generally to robust split inteins. The split inteins described herein are active over a large temperature range, including temperatures as low as 0 DEG C, over a wide pH range, and in the presence of chaotropic salts. The split inteins also show high tolerance to sequence variability in fused heterologous polypeptides and therefore are useful in protein purification and engineering techniques.</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2014</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20140917&DB=EPODOC&CC=CN&NR=104053779A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20140917&DB=EPODOC&CC=CN&NR=104053779A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>MARCO FELIU DIDAC</creatorcontrib><creatorcontrib>SCHMIDT STEFAN ROBERT</creatorcontrib><creatorcontrib>CARVAJAL VALLEJOS PATRICIA KARINA</creatorcontrib><creatorcontrib>PALLISSE BERGWERF ROSER</creatorcontrib><title>Split inteins and uses thereof</title><description>The present invention relates generally to robust split inteins. The split inteins described herein are active over a large temperature range, including temperatures as low as 0 DEG C, over a wide pH range, and in the presence of chaotropic salts. The split inteins also show high tolerance to sequence variability in fused heterologous polypeptides and therefore are useful in protein purification and engineering techniques.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2014</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZJALLsjJLFHIzCtJzcwrVkjMS1EoLU4tVijJSC1KzU_jYWBNS8wpTuWF0twMim6uIc4euqkF-fGpxQWJyal5qSXxzn6GBiYGpsbm5paOxsSoAQDxDiRz</recordid><startdate>20140917</startdate><enddate>20140917</enddate><creator>MARCO FELIU DIDAC</creator><creator>SCHMIDT STEFAN ROBERT</creator><creator>CARVAJAL VALLEJOS PATRICIA KARINA</creator><creator>PALLISSE BERGWERF ROSER</creator><scope>EVB</scope></search><sort><creationdate>20140917</creationdate><title>Split inteins and uses thereof</title><author>MARCO FELIU DIDAC ; SCHMIDT STEFAN ROBERT ; CARVAJAL VALLEJOS PATRICIA KARINA ; PALLISSE BERGWERF ROSER</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN104053779A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2014</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>MARCO FELIU DIDAC</creatorcontrib><creatorcontrib>SCHMIDT STEFAN ROBERT</creatorcontrib><creatorcontrib>CARVAJAL VALLEJOS PATRICIA KARINA</creatorcontrib><creatorcontrib>PALLISSE BERGWERF ROSER</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>MARCO FELIU DIDAC</au><au>SCHMIDT STEFAN ROBERT</au><au>CARVAJAL VALLEJOS PATRICIA KARINA</au><au>PALLISSE BERGWERF ROSER</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Split inteins and uses thereof</title><date>2014-09-17</date><risdate>2014</risdate><abstract>The present invention relates generally to robust split inteins. The split inteins described herein are active over a large temperature range, including temperatures as low as 0 DEG C, over a wide pH range, and in the presence of chaotropic salts. The split inteins also show high tolerance to sequence variability in fused heterologous polypeptides and therefore are useful in protein purification and engineering techniques.</abstract><oa>free_for_read</oa></addata></record> |
fulltext | fulltext_linktorsrc |
identifier | |
ispartof | |
issn | |
language | chi ; eng |
recordid | cdi_epo_espacenet_CN104053779A |
source | esp@cenet |
subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
title | Split inteins and uses thereof |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T16%3A31%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=MARCO%20FELIU%20DIDAC&rft.date=2014-09-17&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN104053779A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |