Molecular biological method for quickly identifying gender of scandent hop
The invention relates to a molecular biological method for identifying gender of scandent hop, belonging to the technical field of the molecular biology. The specific implementing process is as follows: a designed specific primer sequence is utilized and a scandent hop individual genome DNA (deoxyri...
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| creator | LU LONGDOU LI SHUFEN WANG LIANJUN GAO WUJUN DENG CHUANLIANG ZHANG GUOJUN |
| description | The invention relates to a molecular biological method for identifying gender of scandent hop, belonging to the technical field of the molecular biology. The specific implementing process is as follows: a designed specific primer sequence is utilized and a scandent hop individual genome DNA (deoxyribonucleic acid) is used as a template for PCR (Polymerase Chain Reaction) amplification, the reaction system is 25muL, the reaction compositions are as follows: 1U of TaqDNA polymerase, 2muL of dNTP (diethyl-nitrophenyl thiophosphate) and 100-200ng of genome DNA, the PCR amplification is performed on a PCR instrument, the electrophoretic separation is performed to the amplified product with 1.5% of sepharose gel, ethidium bromide is used for dying, the process is observed and recorded by photograph under a gel-imaging system, DL2000 marker is used as a molecular weight mark, the scandent hop with an objective stripe with size of 153bp is male, and the scandent hop without the stripe is female. The SCAR (sequence ch |
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The specific implementing process is as follows: a designed specific primer sequence is utilized and a scandent hop individual genome DNA (deoxyribonucleic acid) is used as a template for PCR (Polymerase Chain Reaction) amplification, the reaction system is 25muL, the reaction compositions are as follows: 1U of TaqDNA polymerase, 2muL of dNTP (diethyl-nitrophenyl thiophosphate) and 100-200ng of genome DNA, the PCR amplification is performed on a PCR instrument, the electrophoretic separation is performed to the amplified product with 1.5% of sepharose gel, ethidium bromide is used for dying, the process is observed and recorded by photograph under a gel-imaging system, DL2000 marker is used as a molecular weight mark, the scandent hop with an objective stripe with size of 153bp is male, and the scandent hop without the stripe is female. 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The specific implementing process is as follows: a designed specific primer sequence is utilized and a scandent hop individual genome DNA (deoxyribonucleic acid) is used as a template for PCR (Polymerase Chain Reaction) amplification, the reaction system is 25muL, the reaction compositions are as follows: 1U of TaqDNA polymerase, 2muL of dNTP (diethyl-nitrophenyl thiophosphate) and 100-200ng of genome DNA, the PCR amplification is performed on a PCR instrument, the electrophoretic separation is performed to the amplified product with 1.5% of sepharose gel, ethidium bromide is used for dying, the process is observed and recorded by photograph under a gel-imaging system, DL2000 marker is used as a molecular weight mark, the scandent hop with an objective stripe with size of 153bp is male, and the scandent hop without the stripe is female. 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The specific implementing process is as follows: a designed specific primer sequence is utilized and a scandent hop individual genome DNA (deoxyribonucleic acid) is used as a template for PCR (Polymerase Chain Reaction) amplification, the reaction system is 25muL, the reaction compositions are as follows: 1U of TaqDNA polymerase, 2muL of dNTP (diethyl-nitrophenyl thiophosphate) and 100-200ng of genome DNA, the PCR amplification is performed on a PCR instrument, the electrophoretic separation is performed to the amplified product with 1.5% of sepharose gel, ethidium bromide is used for dying, the process is observed and recorded by photograph under a gel-imaging system, DL2000 marker is used as a molecular weight mark, the scandent hop with an objective stripe with size of 153bp is male, and the scandent hop without the stripe is female. The SCAR (sequence ch</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Molecular biological method for quickly identifying gender of scandent hop |
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