Primer and kit for swine chlamydia Taqman-MGB probe real-time fluorescence quantification PCR detection and detection method

The invention discloses a primer and kit for swine chlamydia (C.cuis) Taqman-MGB probe real-time fluorescence quantification PCR detection and a detection method. According to the detection method, two primers and one MGB probe are designed according to one area of MOMP in a target sequence, and swi...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	WANG GUOMIN, XIAO JINWEN, WANG YU, LI YINGGUO, YANG JUN, NIE FUPING, YUAN ZENGZHUANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention discloses a primer and kit for swine chlamydia (C.cuis) Taqman-MGB probe real-time fluorescence quantification PCR detection and a detection method. According to the detection method, two primers and one MGB probe are designed according to one area of MOMP in a target sequence, and swine chlamydia can be amplified specifically. The kit comprises an amplified reaction liquid tube, a positive control device, a negative control device and sterilization deionized water. By means of the primer and kit for swine chlamydia (C.cuis) Taqman-MGB probe real-time fluorescence quantification PCR detection and the detection method, a goal target sequence can be detected quickly, efficiently, specifically and sensitively through two amplified reactions and signal collection; moreover, operation is simple and convenient, expensive instruments and reagents are not needed, technical requirements for operators are not required, detection cost is low, and detection time is short.