Devitrification method for vitrified regeneration seedlings of cabbage type rape
The invention discloses a devitrification method for vitrified regeneration seedlings of cabbage type rape, and belongs to the technical field of biology. The devitrification method comprises the following steps that: the vitrified regeneration seedlings generated in a tissue culture process are cut...
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| creator | ZHANG JIEFU ZHOU XIAOYING PU HUIMING HU MAOLONG CHEN SONG FU SANXIONG LONG WEIHUA CHEN FENG ZHANG WEI PENG QI GAO JIANQIN QI CUNKOU |
| description | The invention discloses a devitrification method for vitrified regeneration seedlings of cabbage type rape, and belongs to the technical field of biology. The devitrification method comprises the following steps that: the vitrified regeneration seedlings generated in a tissue culture process are cut and separated on a ultra-clean workbench through an aseptic operating platform and then are transferred in an aseptic culture flask without any culture mediums, the aseptic culture flask is sealed and is subjected to hunger (no culture mediums), low temperature (5 DEG C) and strong illumination (100-150 micromole/s/m ) treatment in an illuminating incubator, the treated vitrified seedlings are transferred in a minimal medium to be subjected to subculture, and the subculture conductions are as follows: the illumination is 50 micromole/s/m , and the illumination period is 16 hours in illumination and 8 hours in dark within 3-7 days. According to the devitrification method disclosed by the invention, the vitrified re |
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The devitrification method comprises the following steps that: the vitrified regeneration seedlings generated in a tissue culture process are cut and separated on a ultra-clean workbench through an aseptic operating platform and then are transferred in an aseptic culture flask without any culture mediums, the aseptic culture flask is sealed and is subjected to hunger (no culture mediums), low temperature (5 DEG C) and strong illumination (100-150 micromole/s/m ) treatment in an illuminating incubator, the treated vitrified seedlings are transferred in a minimal medium to be subjected to subculture, and the subculture conductions are as follows: the illumination is 50 micromole/s/m , and the illumination period is 16 hours in illumination and 8 hours in dark within 3-7 days. 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The devitrification method comprises the following steps that: the vitrified regeneration seedlings generated in a tissue culture process are cut and separated on a ultra-clean workbench through an aseptic operating platform and then are transferred in an aseptic culture flask without any culture mediums, the aseptic culture flask is sealed and is subjected to hunger (no culture mediums), low temperature (5 DEG C) and strong illumination (100-150 micromole/s/m ) treatment in an illuminating incubator, the treated vitrified seedlings are transferred in a minimal medium to be subjected to subculture, and the subculture conductions are as follows: the illumination is 50 micromole/s/m , and the illumination period is 16 hours in illumination and 8 hours in dark within 3-7 days. According to the devitrification method disclosed by the invention, the vitrified re</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING |
| title | Devitrification method for vitrified regeneration seedlings of cabbage type rape |
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