Method for extracting methicillin-resistant staphylococcus aureus DNA (Deoxyribonucleic Acid)
The invention provides a method for extracting methicillin-resistant staphylococcus aureus DNA (Deoxyribonucleic Acid). The method comprises the following steps of: preparing a lysis solution (pH 8.2) by the following components in final concentration: 20mM of Tris, 2mM of Na2-EDTA (Ethylene Diamine...
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| creator | ZOU YUHAN TONG PING JIN SHU HUANG DEKUI ZHU QINGHUI |
| description | The invention provides a method for extracting methicillin-resistant staphylococcus aureus DNA (Deoxyribonucleic Acid). The method comprises the following steps of: preparing a lysis solution (pH 8.2) by the following components in final concentration: 20mM of Tris, 2mM of Na2-EDTA (Ethylene Diamine Tetraacetic Acid), 1.0% of Tritonx-100, 20mg/ml of lysozyme, 0.05g/ml-0.15g/ml of chelex100, 90mu g/ml-100mu g/ml of lysostaphin, 1.8mg/ml-2mg/ml of protease K and the balance of sterile water; getting and suspending 5-10 staphylococcus aureus bacterial colonies, which grow on the solid culture medium, in the 100mu l of the lysis solution, centrifuging for 30 minutes at 56 DEG C, 10 minutes at 100 DEG C and 10 minutes at the speed of 12000rpm; and getting and using the liquid supernatant for the PCR (Polymerase Chain Reaction). |
| format | Patent |
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The method comprises the following steps of: preparing a lysis solution (pH 8.2) by the following components in final concentration: 20mM of Tris, 2mM of Na2-EDTA (Ethylene Diamine Tetraacetic Acid), 1.0% of Tritonx-100, 20mg/ml of lysozyme, 0.05g/ml-0.15g/ml of chelex100, 90mu g/ml-100mu g/ml of lysostaphin, 1.8mg/ml-2mg/ml of protease K and the balance of sterile water; getting and suspending 5-10 staphylococcus aureus bacterial colonies, which grow on the solid culture medium, in the 100mu l of the lysis solution, centrifuging for 30 minutes at 56 DEG C, 10 minutes at 100 DEG C and 10 minutes at the speed of 12000rpm; and getting and using the liquid supernatant for the PCR (Polymerase Chain Reaction).</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2013</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20130619&DB=EPODOC&CC=CN&NR=103160499A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76516</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20130619&DB=EPODOC&CC=CN&NR=103160499A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZOU YUHAN</creatorcontrib><creatorcontrib>TONG PING</creatorcontrib><creatorcontrib>JIN SHU</creatorcontrib><creatorcontrib>HUANG DEKUI</creatorcontrib><creatorcontrib>ZHU QINGHUI</creatorcontrib><title>Method for extracting methicillin-resistant staphylococcus aureus DNA (Deoxyribonucleic Acid)</title><description>The invention provides a method for extracting methicillin-resistant staphylococcus aureus DNA (Deoxyribonucleic Acid). The method comprises the following steps of: preparing a lysis solution (pH 8.2) by the following components in final concentration: 20mM of Tris, 2mM of Na2-EDTA (Ethylene Diamine Tetraacetic Acid), 1.0% of Tritonx-100, 20mg/ml of lysozyme, 0.05g/ml-0.15g/ml of chelex100, 90mu g/ml-100mu g/ml of lysostaphin, 1.8mg/ml-2mg/ml of protease K and the balance of sterile water; getting and suspending 5-10 staphylococcus aureus bacterial colonies, which grow on the solid culture medium, in the 100mu l of the lysis solution, centrifuging for 30 minutes at 56 DEG C, 10 minutes at 100 DEG C and 10 minutes at the speed of 12000rpm; and getting and using the liquid supernatant for the PCR (Polymerase Chain Reaction).</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2013</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNirEKwjAQQLs4iPoP56ZDoaUidCyt4mInVynxerUHMReSFNq_N4Mf4PIePN46ed4pjNLDIA5oDk5hYPOGT6yMrDWb1JFnH5QJEGnHRQsK4uRBTY6imraCQ0MyL45fYibUxAgVcn_cJqtBaU-7nzfJ_np51LeUrHTkrUIyFLq6zbMiP2ensqyKf54vOj88eQ</recordid><startdate>20130619</startdate><enddate>20130619</enddate><creator>ZOU YUHAN</creator><creator>TONG PING</creator><creator>JIN SHU</creator><creator>HUANG DEKUI</creator><creator>ZHU QINGHUI</creator><scope>EVB</scope></search><sort><creationdate>20130619</creationdate><title>Method for extracting methicillin-resistant staphylococcus aureus DNA (Deoxyribonucleic Acid)</title><author>ZOU YUHAN ; TONG PING ; JIN SHU ; HUANG DEKUI ; ZHU QINGHUI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN103160499A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2013</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ZOU YUHAN</creatorcontrib><creatorcontrib>TONG PING</creatorcontrib><creatorcontrib>JIN SHU</creatorcontrib><creatorcontrib>HUANG DEKUI</creatorcontrib><creatorcontrib>ZHU QINGHUI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ZOU YUHAN</au><au>TONG PING</au><au>JIN SHU</au><au>HUANG DEKUI</au><au>ZHU QINGHUI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for extracting methicillin-resistant staphylococcus aureus DNA (Deoxyribonucleic Acid)</title><date>2013-06-19</date><risdate>2013</risdate><abstract>The invention provides a method for extracting methicillin-resistant staphylococcus aureus DNA (Deoxyribonucleic Acid). The method comprises the following steps of: preparing a lysis solution (pH 8.2) by the following components in final concentration: 20mM of Tris, 2mM of Na2-EDTA (Ethylene Diamine Tetraacetic Acid), 1.0% of Tritonx-100, 20mg/ml of lysozyme, 0.05g/ml-0.15g/ml of chelex100, 90mu g/ml-100mu g/ml of lysostaphin, 1.8mg/ml-2mg/ml of protease K and the balance of sterile water; getting and suspending 5-10 staphylococcus aureus bacterial colonies, which grow on the solid culture medium, in the 100mu l of the lysis solution, centrifuging for 30 minutes at 56 DEG C, 10 minutes at 100 DEG C and 10 minutes at the speed of 12000rpm; and getting and using the liquid supernatant for the PCR (Polymerase Chain Reaction).</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for extracting methicillin-resistant staphylococcus aureus DNA (Deoxyribonucleic Acid) |
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