Kit for detecting Listeria monocytogenes and staphylococcus aureus, and application thereof

The invention discloses a fluorescence quantitative PCR (polymerase chain reaction) kit for detecting Listeria monocytogenes and staphylococcus aureus, and an application of the kit, wherein the kit comprises TaqDNA polymerase, fluorescence quantitative PCR amplification buffer solution, sterilized de-ionized water, one pair of primers for amplifying Listeria monocytogenes, one TaqMan probe, one pair of primers for amplifying staphylococcus aureus and one TaqMan probe. According to the invention, by utilizing the principle that multiple fluorescences are used for quantitating PCR real-time, and the advantage of high efficiency of PCR technology, high specificity of probe technology, high sensibility and high accuracy of spectrum technology, the aim of detecting Listeria monocytogenes and staphylococcus aureus synchronously and quantitatively is chieved, and assurance for improving food regulation efficiency and level, and preventing food poisoning and foodborne diseases is provided. Meanwhile, the foundation