Extraction method for aniseed genome DNA (Deoxyribonucleic Acid)
The invention provides an extraction method for an aniseed genome DNA (Deoxyribonucleic Acid). The method comprises the following steps of: adding a CTAB (Cetyltrimethyl Ammonium Bromide) extract solution, uniformly mixing and centrifuging to obtain a supernatant A; adding a chloroform solution and...
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| creator | CHEN SHAOYU HE NA BAI PING CHEN HAIYUN LI YONGJIE MAO YUNLING MA TING GENG SHUXIANG WANG YANG ZHANG YANLI WU TAO GU LIPING NING DELU XIAO LIANGJUN |
| description | The invention provides an extraction method for an aniseed genome DNA (Deoxyribonucleic Acid). The method comprises the following steps of: adding a CTAB (Cetyltrimethyl Ammonium Bromide) extract solution, uniformly mixing and centrifuging to obtain a supernatant A; adding a chloroform solution and an isoamyl alcohol solution, which are the same in volume, into the supernatant A, uniformly mixing, centrifuging, then adding the chloroform solution and the isoamyl alcohol solution, which are the same in volume, into the obtained supernatant, uniformly mixing, standing and performing centrifugal separation to obtain a supernatant B; adding absolute ethyl alcohol at the temperature of minus 30 DEG C into the supernatant B and centrifuging to obtain precipitates; purifying the precipitates again by utilizing an adsorption column used in a conventional kit, eluting, standing, centrifuging and returning the obtained eluent to the adsorption column; and centrifuging the obtain liquid, so as to obtain an aniseed genom |
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The method comprises the following steps of: adding a CTAB (Cetyltrimethyl Ammonium Bromide) extract solution, uniformly mixing and centrifuging to obtain a supernatant A; adding a chloroform solution and an isoamyl alcohol solution, which are the same in volume, into the supernatant A, uniformly mixing, centrifuging, then adding the chloroform solution and the isoamyl alcohol solution, which are the same in volume, into the obtained supernatant, uniformly mixing, standing and performing centrifugal separation to obtain a supernatant B; adding absolute ethyl alcohol at the temperature of minus 30 DEG C into the supernatant B and centrifuging to obtain precipitates; purifying the precipitates again by utilizing an adsorption column used in a conventional kit, eluting, standing, centrifuging and returning the obtained eluent to the adsorption column; and centrifuging the obtain liquid, so as to obtain an aniseed genom</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2012</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120822&DB=EPODOC&CC=CN&NR=102643802A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120822&DB=EPODOC&CC=CN&NR=102643802A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CHEN SHAOYU</creatorcontrib><creatorcontrib>HE NA</creatorcontrib><creatorcontrib>BAI PING</creatorcontrib><creatorcontrib>CHEN HAIYUN</creatorcontrib><creatorcontrib>LI YONGJIE</creatorcontrib><creatorcontrib>MAO YUNLING</creatorcontrib><creatorcontrib>MA TING</creatorcontrib><creatorcontrib>GENG SHUXIANG</creatorcontrib><creatorcontrib>WANG YANG</creatorcontrib><creatorcontrib>ZHANG YANLI</creatorcontrib><creatorcontrib>WU TAO</creatorcontrib><creatorcontrib>GU LIPING</creatorcontrib><creatorcontrib>NING DELU</creatorcontrib><creatorcontrib>XIAO LIANGJUN</creatorcontrib><title>Extraction method for aniseed genome DNA (Deoxyribonucleic Acid)</title><description>The invention provides an extraction method for an aniseed genome DNA (Deoxyribonucleic Acid). 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The method comprises the following steps of: adding a CTAB (Cetyltrimethyl Ammonium Bromide) extract solution, uniformly mixing and centrifuging to obtain a supernatant A; adding a chloroform solution and an isoamyl alcohol solution, which are the same in volume, into the supernatant A, uniformly mixing, centrifuging, then adding the chloroform solution and the isoamyl alcohol solution, which are the same in volume, into the obtained supernatant, uniformly mixing, standing and performing centrifugal separation to obtain a supernatant B; adding absolute ethyl alcohol at the temperature of minus 30 DEG C into the supernatant B and centrifuging to obtain precipitates; purifying the precipitates again by utilizing an adsorption column used in a conventional kit, eluting, standing, centrifuging and returning the obtained eluent to the adsorption column; and centrifuging the obtain liquid, so as to obtain an aniseed genom</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Extraction method for aniseed genome DNA (Deoxyribonucleic Acid) |
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