Fusion protein carrying NS3 preponderant area of non-structural protein of bovine viral diarrhea virus as well as recombinant expression method and application thereof
The invention discloses a fusion protein carrying an NS3 preponderant area of a non-structural protein of a bovine viral diarrhea virus (BVDV) as well as a recombinant expression method and application thereof. The fusion protein disclosed by the invention is obtained after the amino (N) end of the...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | WANG LI ZHANG QIMU ZHANG JINGANG YIN HUIQIONG YANG SHU |
| description | The invention discloses a fusion protein carrying an NS3 preponderant area of a non-structural protein of a bovine viral diarrhea virus (BVDV) as well as a recombinant expression method and application thereof. The fusion protein disclosed by the invention is obtained after the amino (N) end of the NS3 preponderant area of the non-structural protein of the BVDV is connected with foldase DsbA. The activated high expression of the intracellular solubility of an NS3 gene can be realized by the fusion of the DsbA after being mutated and the NS3 preponderant area of the non-structural protein of the BVDV, and the expression quantity is greater than 30 percent. In the expression method of the fusion protein, which is disclosed by the invention, a pET-DsbA expression vector carrying a T7 promoter and a DsbA gene is adopted, the cleavage site of thrombin is adopted as the insertion site of a gene of the NS3 preponderant area of the non-structural protein of the BVDV, and the expressed NS3 immunodominance protein of t |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN102517260A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN102517260A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN102517260A3</originalsourceid><addsrcrecordid>eNqNTjsOwjAM7cKAgDuYAyBBETCjCsTEAntlUpdGSu3ISfmciGuSIsTMYD352e8zzF77Llhh8CqRLINB1aflKxxPy0SSF65IkSOgEoLUwMKzELUzsVN0P2G6XORmmeBme76yyalJkrR2ATDAnZzrUclIe7Hcm9IjZYRPg5ZiIxUgp_HeWYOxp2NDSlKPs0GNLtDki6Nsut-di8MsNSwpeDTEFMviuJjnq8UmX8-3y39-3kAsWJo</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Fusion protein carrying NS3 preponderant area of non-structural protein of bovine viral diarrhea virus as well as recombinant expression method and application thereof</title><source>esp@cenet</source><creator>WANG LI ; ZHANG QIMU ; ZHANG JINGANG ; YIN HUIQIONG ; YANG SHU</creator><creatorcontrib>WANG LI ; ZHANG QIMU ; ZHANG JINGANG ; YIN HUIQIONG ; YANG SHU</creatorcontrib><description>The invention discloses a fusion protein carrying an NS3 preponderant area of a non-structural protein of a bovine viral diarrhea virus (BVDV) as well as a recombinant expression method and application thereof. The fusion protein disclosed by the invention is obtained after the amino (N) end of the NS3 preponderant area of the non-structural protein of the BVDV is connected with foldase DsbA. The activated high expression of the intracellular solubility of an NS3 gene can be realized by the fusion of the DsbA after being mutated and the NS3 preponderant area of the non-structural protein of the BVDV, and the expression quantity is greater than 30 percent. In the expression method of the fusion protein, which is disclosed by the invention, a pET-DsbA expression vector carrying a T7 promoter and a DsbA gene is adopted, the cleavage site of thrombin is adopted as the insertion site of a gene of the NS3 preponderant area of the non-structural protein of the BVDV, and the expressed NS3 immunodominance protein of t</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2012</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120627&DB=EPODOC&CC=CN&NR=102517260A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120627&DB=EPODOC&CC=CN&NR=102517260A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WANG LI</creatorcontrib><creatorcontrib>ZHANG QIMU</creatorcontrib><creatorcontrib>ZHANG JINGANG</creatorcontrib><creatorcontrib>YIN HUIQIONG</creatorcontrib><creatorcontrib>YANG SHU</creatorcontrib><title>Fusion protein carrying NS3 preponderant area of non-structural protein of bovine viral diarrhea virus as well as recombinant expression method and application thereof</title><description>The invention discloses a fusion protein carrying an NS3 preponderant area of a non-structural protein of a bovine viral diarrhea virus (BVDV) as well as a recombinant expression method and application thereof. The fusion protein disclosed by the invention is obtained after the amino (N) end of the NS3 preponderant area of the non-structural protein of the BVDV is connected with foldase DsbA. The activated high expression of the intracellular solubility of an NS3 gene can be realized by the fusion of the DsbA after being mutated and the NS3 preponderant area of the non-structural protein of the BVDV, and the expression quantity is greater than 30 percent. In the expression method of the fusion protein, which is disclosed by the invention, a pET-DsbA expression vector carrying a T7 promoter and a DsbA gene is adopted, the cleavage site of thrombin is adopted as the insertion site of a gene of the NS3 preponderant area of the non-structural protein of the BVDV, and the expressed NS3 immunodominance protein of t</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2012</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNTjsOwjAM7cKAgDuYAyBBETCjCsTEAntlUpdGSu3ISfmciGuSIsTMYD352e8zzF77Llhh8CqRLINB1aflKxxPy0SSF65IkSOgEoLUwMKzELUzsVN0P2G6XORmmeBme76yyalJkrR2ATDAnZzrUclIe7Hcm9IjZYRPg5ZiIxUgp_HeWYOxp2NDSlKPs0GNLtDki6Nsut-di8MsNSwpeDTEFMviuJjnq8UmX8-3y39-3kAsWJo</recordid><startdate>20120627</startdate><enddate>20120627</enddate><creator>WANG LI</creator><creator>ZHANG QIMU</creator><creator>ZHANG JINGANG</creator><creator>YIN HUIQIONG</creator><creator>YANG SHU</creator><scope>EVB</scope></search><sort><creationdate>20120627</creationdate><title>Fusion protein carrying NS3 preponderant area of non-structural protein of bovine viral diarrhea virus as well as recombinant expression method and application thereof</title><author>WANG LI ; ZHANG QIMU ; ZHANG JINGANG ; YIN HUIQIONG ; YANG SHU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN102517260A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2012</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WANG LI</creatorcontrib><creatorcontrib>ZHANG QIMU</creatorcontrib><creatorcontrib>ZHANG JINGANG</creatorcontrib><creatorcontrib>YIN HUIQIONG</creatorcontrib><creatorcontrib>YANG SHU</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WANG LI</au><au>ZHANG QIMU</au><au>ZHANG JINGANG</au><au>YIN HUIQIONG</au><au>YANG SHU</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Fusion protein carrying NS3 preponderant area of non-structural protein of bovine viral diarrhea virus as well as recombinant expression method and application thereof</title><date>2012-06-27</date><risdate>2012</risdate><abstract>The invention discloses a fusion protein carrying an NS3 preponderant area of a non-structural protein of a bovine viral diarrhea virus (BVDV) as well as a recombinant expression method and application thereof. The fusion protein disclosed by the invention is obtained after the amino (N) end of the NS3 preponderant area of the non-structural protein of the BVDV is connected with foldase DsbA. The activated high expression of the intracellular solubility of an NS3 gene can be realized by the fusion of the DsbA after being mutated and the NS3 preponderant area of the non-structural protein of the BVDV, and the expression quantity is greater than 30 percent. In the expression method of the fusion protein, which is disclosed by the invention, a pET-DsbA expression vector carrying a T7 promoter and a DsbA gene is adopted, the cleavage site of thrombin is adopted as the insertion site of a gene of the NS3 preponderant area of the non-structural protein of the BVDV, and the expressed NS3 immunodominance protein of t</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN102517260A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Fusion protein carrying NS3 preponderant area of non-structural protein of bovine viral diarrhea virus as well as recombinant expression method and application thereof |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T13%3A28%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=WANG%20LI&rft.date=2012-06-27&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN102517260A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |