Aspergillus niger strain capable of producing glucose oxidase and application thereof
The invention discloses an Aspergillus niger strain capable of producing glucose oxidase and application thereof, belonging to the technical field of microbes. The invention obtains an Aspergillus niger strain through screening, and the activity of glucose oxidase can be up to 2.2 UmL after the stra...
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| creator | GU LEI SHEN YINA LIU WEI ZHANG JUAN CHEN JIAN DU GUOCHENG FEI LIJIE |
| description | The invention discloses an Aspergillus niger strain capable of producing glucose oxidase and application thereof, belonging to the technical field of microbes. The invention obtains an Aspergillus niger strain through screening, and the activity of glucose oxidase can be up to 2.2 UmL after the strain is fermented for 96 hours; the strain was preserved by China Center for Type Culture Collection on August 16th, 2011; the preservation number is CCTCC NO:M2011291; and the taxonomic name is Aspergillus niger BBE11721. The Aspergillus niger strain disclosed by the invention can produce glucose oxidase at a high yield, and the yield can be up to 4.6 UmL after optimization and flask shaking; the glucose oxidase secreted by the strain can tolerate higher temperature; the enzyme activity is not lost after a coarse enzyme liquid is kept at 40-45 DEG C for 60 minutes; and the enzyme activity is reduced to 65% of the original enzyme activity after the coarse enzyme liquid is kept at 60 DEG C for 30 minutes, and |
| format | Patent |
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The invention obtains an Aspergillus niger strain through screening, and the activity of glucose oxidase can be up to 2.2 UmL<-1> after the strain is fermented for 96 hours; the strain was preserved by China Center for Type Culture Collection on August 16th, 2011; the preservation number is CCTCC NO:M2011291; and the taxonomic name is Aspergillus niger BBE11721. The Aspergillus niger strain disclosed by the invention can produce glucose oxidase at a high yield, and the yield can be up to 4.6 UmL<-1> after optimization and flask shaking; the glucose oxidase secreted by the strain can tolerate higher temperature; the enzyme activity is not lost after a coarse enzyme liquid is kept at 40-45 DEG C for 60 minutes; and the enzyme activity is reduced to 65% of the original enzyme activity after the coarse enzyme liquid is kept at 60 DEG C for 30 minutes, and</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2012</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120321&DB=EPODOC&CC=CN&NR=102382773A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,782,887,25573,76557</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120321&DB=EPODOC&CC=CN&NR=102382773A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>GU LEI</creatorcontrib><creatorcontrib>SHEN YINA</creatorcontrib><creatorcontrib>LIU WEI</creatorcontrib><creatorcontrib>ZHANG JUAN</creatorcontrib><creatorcontrib>CHEN JIAN</creatorcontrib><creatorcontrib>DU GUOCHENG</creatorcontrib><creatorcontrib>FEI LIJIE</creatorcontrib><title>Aspergillus niger strain capable of producing glucose oxidase and application thereof</title><description>The invention discloses an Aspergillus niger strain capable of producing glucose oxidase and application thereof, belonging to the technical field of microbes. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Aspergillus niger strain capable of producing glucose oxidase and application thereof |
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