Expression vector for homologous expression of xylanase gene xynB in Aspergillus niger, genetic engineering bacterial strain and application of genetic engineering bacterial strain
The invention relates to an expression vector for homologous expression of the xylanase gene xynB in the Aspergillus niger. The invention provides the expression vector for homologous expression of the xylanase gene xynB in the Aspergillus niger, the expression vector is constructed by inserting the...
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| creator | YAN ZUWEI BAI AIZHI LIANG YUNZHANG LI QIANZHONG |
| description | The invention relates to an expression vector for homologous expression of the xylanase gene xynB in the Aspergillus niger. The invention provides the expression vector for homologous expression of the xylanase gene xynB in the Aspergillus niger, the expression vector is constructed by inserting the xylanase gene xynB and a non-coding sequence containing some promoter sequences into a vector pBs-T, wherein the non-coding sequence is located at the 5' end of the gene xynB, and thenucleotide sequences of the xylanase gene xynB and the non-coding sequence containing some promoter sequences are shown in the SEQ ID NO.1. The invention further provides a genetic engineering bacterial strain capable of producing the xylanase, wherein the genetic engineering bacterial strain is obtained by converting the expression vector into the Aspergillus niger A327. The invention also provides a genetic engineering bacterial strain capable of highly producing the xylanase, wherein the enzyme production activity is 11000IU/mL (ta |
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The invention provides the expression vector for homologous expression of the xylanase gene xynB in the Aspergillus niger, the expression vector is constructed by inserting the xylanase gene xynB and a non-coding sequence containing some promoter sequences into a vector pBs-T, wherein the non-coding sequence is located at the 5' end of the gene xynB, and thenucleotide sequences of the xylanase gene xynB and the non-coding sequence containing some promoter sequences are shown in the SEQ ID NO.1. The invention further provides a genetic engineering bacterial strain capable of producing the xylanase, wherein the genetic engineering bacterial strain is obtained by converting the expression vector into the Aspergillus niger A327. The invention also provides a genetic engineering bacterial strain capable of highly producing the xylanase, wherein the enzyme production activity is 11000IU/mL (ta</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2012</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120229&DB=EPODOC&CC=CN&NR=102363788A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120229&DB=EPODOC&CC=CN&NR=102363788A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YAN ZUWEI</creatorcontrib><creatorcontrib>BAI AIZHI</creatorcontrib><creatorcontrib>LIANG YUNZHANG</creatorcontrib><creatorcontrib>LI QIANZHONG</creatorcontrib><title>Expression vector for homologous expression of xylanase gene xynB in Aspergillus niger, genetic engineering bacterial strain and application of genetic engineering bacterial strain</title><description>The invention relates to an expression vector for homologous expression of the xylanase gene xynB in the Aspergillus niger. The invention provides the expression vector for homologous expression of the xylanase gene xynB in the Aspergillus niger, the expression vector is constructed by inserting the xylanase gene xynB and a non-coding sequence containing some promoter sequences into a vector pBs-T, wherein the non-coding sequence is located at the 5' end of the gene xynB, and thenucleotide sequences of the xylanase gene xynB and the non-coding sequence containing some promoter sequences are shown in the SEQ ID NO.1. The invention further provides a genetic engineering bacterial strain capable of producing the xylanase, wherein the genetic engineering bacterial strain is obtained by converting the expression vector into the Aspergillus niger A327. The invention also provides a genetic engineering bacterial strain capable of highly producing the xylanase, wherein the enzyme production activity is 11000IU/mL (ta</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2012</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjbEOwjAMRLswIOAfzA4SUAm6lqqIiYm9MsENkYITxQGV_-IDCagSa4eTffI73zh7150PJGIcw5NUdAHapJu7O-u0ewjQH3AtdC-LjEKgiSk53oNhKMVT0MbaxLPRFBa_ezQKiLVhomBYwwVVTBtakBgw5ZCvgN5bozD2BUNy02zUohWa9XOSzQ_1uTouybuGxKP6Pmmq03q1ybf5rijKfAjzAdCbXgo</recordid><startdate>20120229</startdate><enddate>20120229</enddate><creator>YAN ZUWEI</creator><creator>BAI AIZHI</creator><creator>LIANG YUNZHANG</creator><creator>LI QIANZHONG</creator><scope>EVB</scope></search><sort><creationdate>20120229</creationdate><title>Expression vector for homologous expression of xylanase gene xynB in Aspergillus niger, genetic engineering bacterial strain and application of genetic engineering bacterial strain</title><author>YAN ZUWEI ; BAI AIZHI ; LIANG YUNZHANG ; LI QIANZHONG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN102363788A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2012</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>YAN ZUWEI</creatorcontrib><creatorcontrib>BAI AIZHI</creatorcontrib><creatorcontrib>LIANG YUNZHANG</creatorcontrib><creatorcontrib>LI QIANZHONG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YAN ZUWEI</au><au>BAI AIZHI</au><au>LIANG YUNZHANG</au><au>LI QIANZHONG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Expression vector for homologous expression of xylanase gene xynB in Aspergillus niger, genetic engineering bacterial strain and application of genetic engineering bacterial strain</title><date>2012-02-29</date><risdate>2012</risdate><abstract>The invention relates to an expression vector for homologous expression of the xylanase gene xynB in the Aspergillus niger. The invention provides the expression vector for homologous expression of the xylanase gene xynB in the Aspergillus niger, the expression vector is constructed by inserting the xylanase gene xynB and a non-coding sequence containing some promoter sequences into a vector pBs-T, wherein the non-coding sequence is located at the 5' end of the gene xynB, and thenucleotide sequences of the xylanase gene xynB and the non-coding sequence containing some promoter sequences are shown in the SEQ ID NO.1. The invention further provides a genetic engineering bacterial strain capable of producing the xylanase, wherein the genetic engineering bacterial strain is obtained by converting the expression vector into the Aspergillus niger A327. The invention also provides a genetic engineering bacterial strain capable of highly producing the xylanase, wherein the enzyme production activity is 11000IU/mL (ta</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Expression vector for homologous expression of xylanase gene xynB in Aspergillus niger, genetic engineering bacterial strain and application of genetic engineering bacterial strain |
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