Method for purifying genomic deoxyribonucleic acid (DNA) of plant tissues by using goldmag particles

Method for purifying genomic deoxyribonucleic acid (DNA) of plant tissues by using goldmag particles

The invention aims to provide a method for simply and quickly purifying genomic deoxyribonucleic acid (DNA) of plant tissues by using a magnetic nano composite material. The method comprises the following steps of: performing sample lysis by using two kinds of lysis solution, wherein the first lysis...

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Hauptverfasser: ZHANG JINGGE, ZHAO WENCAO, CUI YALI, WEI XUXU, CHAO XU, LI TANJIE
Format: Patent
Sprache:chi ; eng
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BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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creator ZHANG JINGGE
ZHAO WENCAO
CUI YALI
WEI XUXU
CHAO XU
LI TANJIE
description The invention aims to provide a method for simply and quickly purifying genomic deoxyribonucleic acid (DNA) of plant tissues by using a magnetic nano composite material. The method comprises the following steps of: performing sample lysis by using two kinds of lysis solution, wherein the first lysis solution comprises PVP (poly vinyl pyrrolidone) with mass-to-volume ratio of 2 to 5 percent, 2 to 5 percent of CTAB (cetyl trimethyl ammonium bromide), and beta-mercaptoethanol with volume fraction of 0.5 to 2 percent, and the second lysis solution comprises 3 to 6M of guanidine hydrochloride and Triton X-100 with volume fraction of 2 to 5 percent; blending, and performing full lysis for 5 to 30 min in a water bath; and adding 10 to 50 microliters of 10 mg/ml RNase solution, and thus obtainingthe sample lysis solution with the genomic DNA. The purification method is easy to operate, has quick purification process, and has the advantages of high purification rate, high DNA integrity, high purity and the like. Accor
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Method for purifying genomic deoxyribonucleic acid (DNA) of plant tissues by using goldmag particles
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