Method for producing recombinant human bone morphogenetic protein-2 mature peptide
The invention relates to a method for producing recombinant human bone morphogenetic protein-2 mature peptide. The method comprises the following steps: sampling a recombinant human bone morphogenetic protein-2 mature peptide solution with good renaturation into a balanced hydrophobic chromatography...
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| creator | ZHU QINGYU GUO WANGMING WANG TONGYING |
| description | The invention relates to a method for producing recombinant human bone morphogenetic protein-2 mature peptide. The method comprises the following steps: sampling a recombinant human bone morphogenetic protein-2 mature peptide solution with good renaturation into a balanced hydrophobic chromatography column, then performing a stepped-gradient elution which gradually reduces the salinity by an elution buffer solution, and collecting target peaks. In order to further enhance the protein purity of the target peaks, the purification method can be a multi-step hydrophobic interaction chromatography or be used by combining with the ion exchange chromatography. The method has the advantages of simple operation, lower cost, higher purification yield (more than 20%), higher purity (SDS-PAGE, HPLC and HPCE are greater than 97%) and the like, and is suitable for producing the recombinant human bone morphogenetic protein family. |
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The method comprises the following steps: sampling a recombinant human bone morphogenetic protein-2 mature peptide solution with good renaturation into a balanced hydrophobic chromatography column, then performing a stepped-gradient elution which gradually reduces the salinity by an elution buffer solution, and collecting target peaks. In order to further enhance the protein purity of the target peaks, the purification method can be a multi-step hydrophobic interaction chromatography or be used by combining with the ion exchange chromatography. 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The method comprises the following steps: sampling a recombinant human bone morphogenetic protein-2 mature peptide solution with good renaturation into a balanced hydrophobic chromatography column, then performing a stepped-gradient elution which gradually reduces the salinity by an elution buffer solution, and collecting target peaks. In order to further enhance the protein purity of the target peaks, the purification method can be a multi-step hydrophobic interaction chromatography or be used by combining with the ion exchange chromatography. The method has the advantages of simple operation, lower cost, higher purification yield (more than 20%), higher purity (SDS-PAGE, HPLC and HPCE are greater than 97%) and the like, and is suitable for producing the recombinant human bone morphogenetic protein family.</abstract><oa>free_for_read</oa></addata></record> |
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| title | Method for producing recombinant human bone morphogenetic protein-2 mature peptide |
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