Gene chip and applications thereof in detection of aquatic pathogenic microorganism
The invention relates to a gene chip in the detection of aquatic pathogenic microorganism, a preparation method and applications thereof in the detection of aquatic pathogenic microorganism. A conserved sequence selecting from a target pathogenic microorganism gene is fixed on a solid-phase carrier...
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| creator | XU BAOQING FENG XIAOYU GUO SHUIRONG LIN QICUN LIU KAI CAI LIJUAN |
| description | The invention relates to a gene chip in the detection of aquatic pathogenic microorganism, a preparation method and applications thereof in the detection of aquatic pathogenic microorganism. A conserved sequence selecting from a target pathogenic microorganism gene is fixed on a solid-phase carrier as an oligonucleotide probe, in the proper hybridization condition, a marked sample to be detected is added for reaction, and the corresponding aquatic pathogenic microorganism can be identified according to a probe display signal on the gene chip. The gene chip can fast detect the aquatic pathogenic microorganism such as aeromonas hydrophila, edwardsiella tarda, staphylococcus aureus, vibrio parahaemolyticus, listeria monocytogenes, salmonella typhimurium, shigella and the like, thus being high in detection sensitivity, and strong in specificity and greatly shortening the detection period. |
| format | Patent |
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A conserved sequence selecting from a target pathogenic microorganism gene is fixed on a solid-phase carrier as an oligonucleotide probe, in the proper hybridization condition, a marked sample to be detected is added for reaction, and the corresponding aquatic pathogenic microorganism can be identified according to a probe display signal on the gene chip. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Gene chip and applications thereof in detection of aquatic pathogenic microorganism |
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