Extraction and purification method of TSHR (Thyroid Stimulating Hormone Receptor)

The invention discloses an extraction and purification method of TSHR (Thyroid Stimulating Hormone Receptor). The extraction method comprises the following steps of: (1) adding a buffer solution (A) to pork thyroid tissue with fat and connective tissues removed and grinding with a homogenizer; (2) c...

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Hauptverfasser:	LIU HEJUN, LI JIE, CHEN LUCHI, WANG KAI, YANG BIN, WANG LIKAI, PAN XUEJI, ZHANG CHAO, WANG YICHEN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	CHEMISTRY METALLURGY ORGANIC CHEMISTRY PEPTIDES
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creator	LIU HEJUN LI JIE CHEN LUCHI WANG KAI YANG BIN WANG LIKAI PAN XUEJI ZHANG CHAO WANG YICHEN
description	The invention discloses an extraction and purification method of TSHR (Thyroid Stimulating Hormone Receptor). The extraction method comprises the following steps of: (1) adding a buffer solution (A) to pork thyroid tissue with fat and connective tissues removed and grinding with a homogenizer; (2) centrifuging; (3) taking supernatant, centrifuging, discarding the supernatant, adding a buffer solution (B) to sediment, uniformly shaking, centrifuging and discarding the supernatant; and (4) adding a buffer solution (C) to the sediment, extracting, uniformly shaking, centrifuging, discarding the sediment to obtain a rough TSHR solution and freezing for spare use. The invention extracts and purifies the most important component TSH receptor in the TSHR detection, greatly regulates and improvesthe technical process of extraction and purification and can favorably keep the biological activity of the pork TSH receptor. The extraction and purification method has high yield and overcomes the difficulties that a traditi
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title	Extraction and purification method of TSHR (Thyroid Stimulating Hormone Receptor)
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