Inducing method for differentiating umbilical cord mesenchymal stem cells into nerve cells

Inducing method for differentiating umbilical cord mesenchymal stem cells into nerve cells

The invention relates to the field of biotechnology, in particular to an inducing method for differentiating umbilical cord mesenchymal stem cells into nerve cells, which comprises the following steps of: cultivating the separated umbilical cord mesenchymal stem cells in an MSC culture medium to spr...

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Hauptverfasser: LIU DONG, LI DONG, FAN FENGQUN
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Sprache:chi ; eng
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BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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creator LIU DONG
LI DONG
FAN FENGQUN
description The invention relates to the field of biotechnology, in particular to an inducing method for differentiating umbilical cord mesenchymal stem cells into nerve cells, which comprises the following steps of: cultivating the separated umbilical cord mesenchymal stem cells in an MSC culture medium to spread a whole culture dish; cultivating the stably-subculturing mesenchymal stem cells in the culture dish which is spread with the extracellular matrix by means of the MSC culture medium to be 70% confluence; and leading the 70% confluent mesenchymal stem cells to sequentially pass through four inducing culture mediums to cultivate, wherein the four inducing culture mediums comprise the following factors of 5-azacytidine+Pam3CSK4, bFGF+Noggin, bFGF+RA+FGF8+Wnt3a, and Bmp4+Shh+RA+NGF+BDNF. The inducing method has the benefit effects of being free of chemical substances with cytotoxicity, transgenic technology and nerve cell co-cultivation, and being high in differentiating efficiency and shorter in time consumption.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Inducing method for differentiating umbilical cord mesenchymal stem cells into nerve cells
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