Method for inducing sugarcane callus by using Lingfasu
The invention discloses a method for inducing sugarcane callus by using Lingfasu. In the method, an MS is used as a basal culture medium, 0.05 to 0.5 mg.l-1 of Lingfasu is added into the basal culture medium to ensure that the basal culture medium is prepared into an improved type solid culture medi...
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creator | LIU LIMIN DAN MING LAN TAOJU YU KUNXING LIU HONGJIAN FANG FENGXUE YOU JIANHUA LU MANMAN LI SONG XU HONGYUAN LIU XIAOYAN YANG RONGZHONG TAN FANG TANG HONGQIN GONG YINHUA |
description | The invention discloses a method for inducing sugarcane callus by using Lingfasu. In the method, an MS is used as a basal culture medium, 0.05 to 0.5 mg.l-1 of Lingfasu is added into the basal culture medium to ensure that the basal culture medium is prepared into an improved type solid culture medium, and a sugarcane spire slice is used as an explant to perform sterile culture by the book. The method is used for inducing the sugarcane callus, the inducing efficiency is up to 90 to 95 percent, and the callus cannot be browned easily, has a loosen texture, is not hardened, is proliferated quickly and has strong vital force. |
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In the method, an MS is used as a basal culture medium, 0.05 to 0.5 mg.l-1 of Lingfasu is added into the basal culture medium to ensure that the basal culture medium is prepared into an improved type solid culture medium, and a sugarcane spire slice is used as an explant to perform sterile culture by the book. 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In the method, an MS is used as a basal culture medium, 0.05 to 0.5 mg.l-1 of Lingfasu is added into the basal culture medium to ensure that the basal culture medium is prepared into an improved type solid culture medium, and a sugarcane spire slice is used as an explant to perform sterile culture by the book. The method is used for inducing the sugarcane callus, the inducing efficiency is up to 90 to 95 percent, and the callus cannot be browned easily, has a loosen texture, is not hardened, is proliferated quickly and has strong vital force.</abstract><oa>free_for_read</oa></addata></record> |
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subjects | AGRICULTURE ANIMAL HUSBANDRY FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING |
title | Method for inducing sugarcane callus by using Lingfasu |
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