Method for inducing sugarcane callus by using Lingfasu

Method for inducing sugarcane callus by using Lingfasu

The invention discloses a method for inducing sugarcane callus by using Lingfasu. In the method, an MS is used as a basal culture medium, 0.05 to 0.5 mg.l-1 of Lingfasu is added into the basal culture medium to ensure that the basal culture medium is prepared into an improved type solid culture medi...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: LIU LIMIN, DAN MING, LAN TAOJU, YU KUNXING, LIU HONGJIAN, FANG FENGXUE, YOU JIANHUA, LU MANMAN, LI SONG, XU HONGYUAN, LIU XIAOYAN, YANG RONGZHONG, TAN FANG, TANG HONGQIN, GONG YINHUA
Format: Patent
Sprache:chi ; eng
Schlagworte:
AGRICULTURE
ANIMAL HUSBANDRY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
TRAPPING
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Beschreibung
Zusammenfassung:The invention discloses a method for inducing sugarcane callus by using Lingfasu. In the method, an MS is used as a basal culture medium, 0.05 to 0.5 mg.l-1 of Lingfasu is added into the basal culture medium to ensure that the basal culture medium is prepared into an improved type solid culture medium, and a sugarcane spire slice is used as an explant to perform sterile culture by the book. The method is used for inducing the sugarcane callus, the inducing efficiency is up to 90 to 95 percent, and the callus cannot be browned easily, has a loosen texture, is not hardened, is proliferated quickly and has strong vital force.