Method for producing swine fever live vaccine by using porcine kidney cell line (IBRS-2) and product thereof
The invention discloses a method for producing a swine fever live vaccine by using a porcine kidney cell line (IBRS-2). The method comprises the following steps of: (1) selecting a porcine kidney cell line (IBRS-2) as a cell for producing the vaccine; (2) carrying out sub-culturing on the cell for p...
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| creator | LIN XUYE NING YIBAO WU WENFU YOU QIYOU LIU QIUYAN YANG AOBING QIU CHENGWEN LIU FALIAN CEN XIAOQING ZHANG YUJIN REN XIANGYANG |
| description | The invention discloses a method for producing a swine fever live vaccine by using a porcine kidney cell line (IBRS-2). The method comprises the following steps of: (1) selecting a porcine kidney cell line (IBRS-2) as a cell for producing the vaccine; (2) carrying out sub-culturing on the cell for producing the vaccine; (3) reproducing virus cell seeds; (4) reproducing venom for producing the vaccine; and (5) preparing the vaccine, and performing split charging and freeze-drying. The method of the invention has the advantages of simple and stable production process, easy operation, high content of virus, small difference between bitches, and controllable quality, and capability of improving the quality and yield of the vaccine. The swine fever live vaccine produced by the method of the invention has high safety and high immune effect, and has a complete immune protection function on the attacks of the strong swine fever viruses. |
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The method comprises the following steps of: (1) selecting a porcine kidney cell line (IBRS-2) as a cell for producing the vaccine; (2) carrying out sub-culturing on the cell for producing the vaccine; (3) reproducing virus cell seeds; (4) reproducing venom for producing the vaccine; and (5) preparing the vaccine, and performing split charging and freeze-drying. The method of the invention has the advantages of simple and stable production process, easy operation, high content of virus, small difference between bitches, and controllable quality, and capability of improving the quality and yield of the vaccine. The swine fever live vaccine produced by the method of the invention has high safety and high immune effect, and has a complete immune protection function on the attacks of the strong swine fever viruses.</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2012</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120620&DB=EPODOC&CC=CN&NR=101850115B$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76516</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120620&DB=EPODOC&CC=CN&NR=101850115B$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIN XUYE</creatorcontrib><creatorcontrib>NING YIBAO</creatorcontrib><creatorcontrib>WU WENFU</creatorcontrib><creatorcontrib>YOU QIYOU</creatorcontrib><creatorcontrib>LIU QIUYAN</creatorcontrib><creatorcontrib>YANG AOBING</creatorcontrib><creatorcontrib>QIU CHENGWEN</creatorcontrib><creatorcontrib>LIU FALIAN</creatorcontrib><creatorcontrib>CEN XIAOQING</creatorcontrib><creatorcontrib>ZHANG YUJIN</creatorcontrib><creatorcontrib>REN XIANGYANG</creatorcontrib><title>Method for producing swine fever live vaccine by using porcine kidney cell line (IBRS-2) and product thereof</title><description>The invention discloses a method for producing a swine fever live vaccine by using a porcine kidney cell line (IBRS-2). The method comprises the following steps of: (1) selecting a porcine kidney cell line (IBRS-2) as a cell for producing the vaccine; (2) carrying out sub-culturing on the cell for producing the vaccine; (3) reproducing virus cell seeds; (4) reproducing venom for producing the vaccine; and (5) preparing the vaccine, and performing split charging and freeze-drying. The method of the invention has the advantages of simple and stable production process, easy operation, high content of virus, small difference between bitches, and controllable quality, and capability of improving the quality and yield of the vaccine. 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The method comprises the following steps of: (1) selecting a porcine kidney cell line (IBRS-2) as a cell for producing the vaccine; (2) carrying out sub-culturing on the cell for producing the vaccine; (3) reproducing virus cell seeds; (4) reproducing venom for producing the vaccine; and (5) preparing the vaccine, and performing split charging and freeze-drying. The method of the invention has the advantages of simple and stable production process, easy operation, high content of virus, small difference between bitches, and controllable quality, and capability of improving the quality and yield of the vaccine. The swine fever live vaccine produced by the method of the invention has high safety and high immune effect, and has a complete immune protection function on the attacks of the strong swine fever viruses.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | Method for producing swine fever live vaccine by using porcine kidney cell line (IBRS-2) and product thereof |
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