Fast typing detection method of high-flux visualized HPV (Human Papilloma Virus) gene chip

The invention relates to a fast typing detection method of a high-flux visualized HPV (Human Papilloma Virus). In the method, a DNA probe is marked by using colloidal gold, and a gold signal is improved by silver dying and judged directly and visually without expensive signal detection equipment. Th...

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Bibliographische Detailangaben
Hauptverfasser: LI ZHENG, WANG XIAOQIANG, DANG QIANLI, CHEN CHAO, DANG ERLE, GUO SEN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a fast typing detection method of a high-flux visualized HPV (Human Papilloma Virus). In the method, a DNA probe is marked by using colloidal gold, and a gold signal is improved by silver dying and judged directly and visually without expensive signal detection equipment. The invention has simple and convenient operation, time saving, high sensitivity, stable detection result and good accuracy and solves the defect that a traditional detection result can not be directly viewed by naked eyes but needs a corresponding instrument for detection. A gene chip integration system for HPV typing detection can rapidly investigate infected HPV hypotypes, and detection objects can cover a plurality of domestic common high-incidence and serious-damage HPV hypotypes. By the chip design, a plurality of high-flux clinical detection requirements of a plurality of patients can be met through one examination. The method can be widely used for clinical diagnosis and female physical examination or the scr