Method for constructing human monoclonal antibody fragment complex
The invention relates to a method for constructing a human monoclonal antibody fragment complex, which comprises the following steps: (1) separating B cells from human whole blood and amplifying human antibody heavy chain and light chain gene segments from the micro B cells of a human body; (2) obta...
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| creator | ZHANG FENGWU WU JIANHUA WU HONGFEI SHEN JUYING ZHANG ZUCHUAN GONG ZUXUN YAO YAMEI |
| description | The invention relates to a method for constructing a human monoclonal antibody fragment complex, which comprises the following steps: (1) separating B cells from human whole blood and amplifying human antibody heavy chain and light chain gene segments from the micro B cells of a human body; (2) obtaining light chain and heavy chain functional segments of the human antibody through an RT-PCR method from the gene segments obtained in the step (1), respectively cloning the light chain and heavy chain functional segments into expression vectors pCABHCV and pCABLCV; and (3) doubly transforming colibacillus JM109 or BL21 through the pCABHCV and the pCABLCV, and after the human antibody heavy chain or light chain segments are expressed, simultaneously secreting in a culture solution supernatant and forming the human monoclonal antibody fragment complex. Compared with the prior art, the method for constructing the human monoclonal antibody fragment complex has the advantages of advanced and unique process and simple |
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Compared with the prior art, the method for constructing the human monoclonal antibody fragment complex has the advantages of advanced and unique process and simple</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for constructing human monoclonal antibody fragment complex |
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