Method for measuring materials inside human single blood erythrocyte by mocro-fluidic chip chemiluminescence

The invention provides a method for measuring materials inside human single blood erythrocyte by mocro-fluidic chip chemiluminescence, which can analyze the contents of cysteine, glutathione and hemoglobin in a single blood erythrocyte. An electrophoresis chemiluminescence detection system of a mocro-fluidic chip is adopted; five power supply output ends of a high voltage power supply are respectively connected with a buffer solution pool B, a sample pool S, a waste liquie pool SW, a chemiluminescence reagent pool R and a buffer waste liquie pool BW of the mocro-fluidic chip; the mocro-fluidic chip is arranged on an objective lens of a chemiluminescence detector; the chemiluminescence detector is an inverted microscope combined with a photomultiplier; the photomultiplier is electrically connected with a data collector; and the data collector is electrically connected with a computer. The method has high analysis speed, high sensitivity and high resolution.