Enzyme-linked immuno kit and method for detecting bovine lactoferrin by using same

The invention discloses an enzyme-linked immuno kit and a method for detecting bovine lactoferrin by using same, relating to a kit and a method for detecting bovine lactoferrin by using the kit. The invention solves the problems of the existing method for detecting bovine lactoferrin that the detection cost is higher, and the operation is complicated. The enzyme-linked immuno kit comprises an enzyme-linked plate, bovine lactoferrin standard substance, sample diluent, detection solution, substrate solution, cleaning solution and stop solution; the detection method mainly comprises the steps ofpreparing solution, combining an antibody on the enzyme-linked plate, closing the enzyme-linked plate, fixing the standard substance and the sample to be detected on the enzyme-linked plate, detectinglight absorption value by a microplate reader, making a standard curve according to detection results, and then calculating the content of the ovine lactoferrin serous of the sample to be detected. The method adopts a working