Magnaporthe grisea exciton CSB I gene and clone method thereof
The invention discloses CSB I gene of rice blast fungus elicitor and cloning method thereof, including nucleotide sequence of the gene encoding, the sequence of the total length of cDNA as SEQ ID NO: I and amino acid sequence as SEQ ID NO: 2. Primers are designed according to the coding sequence of...
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| creator | JI CHUNYAN WANG ZHENZHONG TONG YINGLIN LI YUNFENG DONG ZHANGYONG |
| description | The invention discloses CSB I gene of rice blast fungus elicitor and cloning method thereof, including nucleotide sequence of the gene encoding, the sequence of the total length of cDNA as SEQ ID NO: I and amino acid sequence as SEQ ID NO: 2. Primers are designed according to the coding sequence of peptide end of the CSB I elicitor which has been measured. Total RNA of physiological race of rice blast fungus ZC13 is taken as template, and first strand of cDNA can be synthesized by RT-PCR method. The deduced cDNA sequence of the CSB I elicitor of physiological race of the rice blast fungus ZC13 can be achieved by utilizing the fast amplified technology to clone. The invention can be the basement of the mechanism of wide and abundant researches on the producing disease assistant activity of the rice induced by the elicitor. Besides, the invention can also be used for research on transgenic breeding of the rice. |
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Primers are designed according to the coding sequence of peptide end of the CSB I elicitor which has been measured. Total RNA of physiological race of rice blast fungus ZC13 is taken as template, and first strand of cDNA can be synthesized by RT-PCR method. The deduced cDNA sequence of the CSB I elicitor of physiological race of the rice blast fungus ZC13 can be achieved by utilizing the fast amplified technology to clone. The invention can be the basement of the mechanism of wide and abundant researches on the producing disease assistant activity of the rice induced by the elicitor. Besides, the invention can also be used for research on transgenic breeding of the rice.</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2008</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20080528&DB=EPODOC&CC=CN&NR=101186917A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76294</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20080528&DB=EPODOC&CC=CN&NR=101186917A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>JI CHUNYAN</creatorcontrib><creatorcontrib>WANG ZHENZHONG</creatorcontrib><creatorcontrib>TONG YINGLIN</creatorcontrib><creatorcontrib>LI YUNFENG</creatorcontrib><creatorcontrib>DONG ZHANGYONG</creatorcontrib><title>Magnaporthe grisea exciton CSB I gene and clone method thereof</title><description>The invention discloses CSB I gene of rice blast fungus elicitor and cloning method thereof, including nucleotide sequence of the gene encoding, the sequence of the total length of cDNA as SEQ ID NO: I and amino acid sequence as SEQ ID NO: 2. Primers are designed according to the coding sequence of peptide end of the CSB I elicitor which has been measured. Total RNA of physiological race of rice blast fungus ZC13 is taken as template, and first strand of cDNA can be synthesized by RT-PCR method. The deduced cDNA sequence of the CSB I elicitor of physiological race of the rice blast fungus ZC13 can be achieved by utilizing the fast amplified technology to clone. The invention can be the basement of the mechanism of wide and abundant researches on the producing disease assistant activity of the rice induced by the elicitor. 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Primers are designed according to the coding sequence of peptide end of the CSB I elicitor which has been measured. Total RNA of physiological race of rice blast fungus ZC13 is taken as template, and first strand of cDNA can be synthesized by RT-PCR method. The deduced cDNA sequence of the CSB I elicitor of physiological race of the rice blast fungus ZC13 can be achieved by utilizing the fast amplified technology to clone. The invention can be the basement of the mechanism of wide and abundant researches on the producing disease assistant activity of the rice induced by the elicitor. Besides, the invention can also be used for research on transgenic breeding of the rice.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Magnaporthe grisea exciton CSB I gene and clone method thereof |
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