Sequential protein isolation and purification schemes by affinity chromatography

The invention discloses methods for sequential protein isolation and purification from a biological sample by affinity chromatography. Affinity chromatography is conducted using ligands or ligand support complexes that selectively and specifically bind to proteins in the biological sample. The ligan...

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Hauptverfasser: BURTON STEVEN JAMES, HAMMOND DAVID JOHN, CHEN DWUN-HOU, BAINES BALDEV, CURLING JOHN, BRYANT CHRISTOPHER, HAYES TIMOTHY KEITH
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creator BURTON STEVEN JAMES
HAMMOND DAVID JOHN
CHEN DWUN-HOU
BAINES BALDEV
CURLING JOHN
BRYANT CHRISTOPHER
HAYES TIMOTHY KEITH
description The invention discloses methods for sequential protein isolation and purification from a biological sample by affinity chromatography. Affinity chromatography is conducted using ligands or ligand support complexes that selectively and specifically bind to proteins in the biological sample. The ligands or ligand support complexes were contacted sequentially in a predetermined order with the biological sample to allow each ligand or ligand-support complex to sequentially bind a protein from the biological sample.
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subjects CHEMISTRY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
title Sequential protein isolation and purification schemes by affinity chromatography
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