METHODS FOR MEASURING POLY A TAIL LENGTH

The present invention provides, among other things, methods of measuring homopolymeric nucleotide lengths in a nucleic acid, including mRNA. In some aspects, provided herein is a method of measuring poly A tail length in mRNA comprising binding of mRNA with a minor-groove binding dye, followed by ri...

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Bibliographische Detailangaben
Hauptverfasser:	DIAS, ANUSHA, DUBINS, JEFFREY S, VARGAS, JOREL E, ABYSALH, JONATHAN
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides, among other things, methods of measuring homopolymeric nucleotide lengths in a nucleic acid, including mRNA. In some aspects, provided herein is a method of measuring poly A tail length in mRNA comprising binding of mRNA with a minor-groove binding dye, followed by ribonuclease digestion and capillary electrophoresis. La présente invention concerne, entre autres, des procédés de mesure de longueurs de nucléotides homopolymériques dans un acide nucléique, comprenant l'ARNm. Dans certains aspects, la présente invention concerne un procédé de mesure de la longueur de queue poly A d'un ARNm, comprenant la liaison de l'ARNm avec un colorant de liaison à petit sillon, puis une digestion de ribonucléase et une électrophorèse capillaire.