FLAVIN-DEPENDENT OXIDASES HAVING CANNABINOID SYNTHASE ACTIVITY
The disclosure relates to a non-natural flavin-dependent oxidase comprising at least one amino acid variation as compared to a wild type flavin-dependent oxidase, wherein the non-natural flavin-dependent oxidase does not comprise a disulfide bond, and wherein the non-natural flavin-dependent oxidase...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Format: | Patent |
| Sprache: | eng ; fre |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | HUDDLESTON, JAMISON PARKER ZHANG, DEQIANG SCHIRMER, ANDREAS W |
| description | The disclosure relates to a non-natural flavin-dependent oxidase comprising at least one amino acid variation as compared to a wild type flavin-dependent oxidase, wherein the non-natural flavin-dependent oxidase does not comprise a disulfide bond, and wherein the non-natural flavin-dependent oxidase is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid. The disclosure also relates to a nucleic acid, an expression construct, and an engineered cell for making the non-natural flavin-dependent oxidase. Also provided are compositions comprising the non-natural flavin-dependent oxidase; isolated non-natural flavin-dependent oxidase and methods of making the same; cell extracts comprising the non-natural flavin-dependent oxidase; and methods of making cannabinoids.
L'invention concerne une oxydase flavine-dépendante non naturelle comprenant au moins une variation d'acide aminé par comparaison avec une oxydase flavine-dépendante de type sauvage, l'oxydase flavine-dépendante non naturelle ne comprenant pas de liaison disulfure, et l'oxydase flavine-dépendante non naturelle étant capable de cyclisation oxydative d'un composé aromatique prénylé en un cannabinoïde. L'invention concerne également un acide nucléique, une construction d'expression et une cellule modifiée pour préparer l'oxydase flavine-dépendante non naturelle. L'invention concerne également des compositions comprenant l'oxydase flavine-dépendante non naturelle; une oxydase flavine-dépendante non naturelle isolée et leurs procédés de fabrication; des extraits cellulaires comprenant l'oxydase flavine-dépendante non naturelle; et des procédés de fabrication de cannabinoïdes. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CA3190913A1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CA3190913A1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CA3190913A13</originalsourceid><addsrcrecordid>eNrjZLBz83EM8_TTdXENcPVzcfULUfCP8HRxDHYNVvAASbgrODv6-Tk6efr5e7ooBEf6hXgAJRUcnUM8wzxDInkYWNMSc4pTeaE0N4OCm2uIs4duakF-fGpxQWJyal5qSbyzo7GhpYGlobGjoTERSgAcnynS</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>FLAVIN-DEPENDENT OXIDASES HAVING CANNABINOID SYNTHASE ACTIVITY</title><source>esp@cenet</source><creator>HUDDLESTON, JAMISON PARKER ; ZHANG, DEQIANG ; SCHIRMER, ANDREAS W</creator><creatorcontrib>HUDDLESTON, JAMISON PARKER ; ZHANG, DEQIANG ; SCHIRMER, ANDREAS W</creatorcontrib><description>The disclosure relates to a non-natural flavin-dependent oxidase comprising at least one amino acid variation as compared to a wild type flavin-dependent oxidase, wherein the non-natural flavin-dependent oxidase does not comprise a disulfide bond, and wherein the non-natural flavin-dependent oxidase is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid. The disclosure also relates to a nucleic acid, an expression construct, and an engineered cell for making the non-natural flavin-dependent oxidase. Also provided are compositions comprising the non-natural flavin-dependent oxidase; isolated non-natural flavin-dependent oxidase and methods of making the same; cell extracts comprising the non-natural flavin-dependent oxidase; and methods of making cannabinoids.
L'invention concerne une oxydase flavine-dépendante non naturelle comprenant au moins une variation d'acide aminé par comparaison avec une oxydase flavine-dépendante de type sauvage, l'oxydase flavine-dépendante non naturelle ne comprenant pas de liaison disulfure, et l'oxydase flavine-dépendante non naturelle étant capable de cyclisation oxydative d'un composé aromatique prénylé en un cannabinoïde. L'invention concerne également un acide nucléique, une construction d'expression et une cellule modifiée pour préparer l'oxydase flavine-dépendante non naturelle. L'invention concerne également des compositions comprenant l'oxydase flavine-dépendante non naturelle; une oxydase flavine-dépendante non naturelle isolée et leurs procédés de fabrication; des extraits cellulaires comprenant l'oxydase flavine-dépendante non naturelle; et des procédés de fabrication de cannabinoïdes.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220310&DB=EPODOC&CC=CA&NR=3190913A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220310&DB=EPODOC&CC=CA&NR=3190913A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HUDDLESTON, JAMISON PARKER</creatorcontrib><creatorcontrib>ZHANG, DEQIANG</creatorcontrib><creatorcontrib>SCHIRMER, ANDREAS W</creatorcontrib><title>FLAVIN-DEPENDENT OXIDASES HAVING CANNABINOID SYNTHASE ACTIVITY</title><description>The disclosure relates to a non-natural flavin-dependent oxidase comprising at least one amino acid variation as compared to a wild type flavin-dependent oxidase, wherein the non-natural flavin-dependent oxidase does not comprise a disulfide bond, and wherein the non-natural flavin-dependent oxidase is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid. The disclosure also relates to a nucleic acid, an expression construct, and an engineered cell for making the non-natural flavin-dependent oxidase. Also provided are compositions comprising the non-natural flavin-dependent oxidase; isolated non-natural flavin-dependent oxidase and methods of making the same; cell extracts comprising the non-natural flavin-dependent oxidase; and methods of making cannabinoids.
L'invention concerne une oxydase flavine-dépendante non naturelle comprenant au moins une variation d'acide aminé par comparaison avec une oxydase flavine-dépendante de type sauvage, l'oxydase flavine-dépendante non naturelle ne comprenant pas de liaison disulfure, et l'oxydase flavine-dépendante non naturelle étant capable de cyclisation oxydative d'un composé aromatique prénylé en un cannabinoïde. L'invention concerne également un acide nucléique, une construction d'expression et une cellule modifiée pour préparer l'oxydase flavine-dépendante non naturelle. L'invention concerne également des compositions comprenant l'oxydase flavine-dépendante non naturelle; une oxydase flavine-dépendante non naturelle isolée et leurs procédés de fabrication; des extraits cellulaires comprenant l'oxydase flavine-dépendante non naturelle; et des procédés de fabrication de cannabinoïdes.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLBz83EM8_TTdXENcPVzcfULUfCP8HRxDHYNVvAASbgrODv6-Tk6efr5e7ooBEf6hXgAJRUcnUM8wzxDInkYWNMSc4pTeaE0N4OCm2uIs4duakF-fGpxQWJyal5qSbyzo7GhpYGlobGjoTERSgAcnynS</recordid><startdate>20220310</startdate><enddate>20220310</enddate><creator>HUDDLESTON, JAMISON PARKER</creator><creator>ZHANG, DEQIANG</creator><creator>SCHIRMER, ANDREAS W</creator><scope>EVB</scope></search><sort><creationdate>20220310</creationdate><title>FLAVIN-DEPENDENT OXIDASES HAVING CANNABINOID SYNTHASE ACTIVITY</title><author>HUDDLESTON, JAMISON PARKER ; ZHANG, DEQIANG ; SCHIRMER, ANDREAS W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CA3190913A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HUDDLESTON, JAMISON PARKER</creatorcontrib><creatorcontrib>ZHANG, DEQIANG</creatorcontrib><creatorcontrib>SCHIRMER, ANDREAS W</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HUDDLESTON, JAMISON PARKER</au><au>ZHANG, DEQIANG</au><au>SCHIRMER, ANDREAS W</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>FLAVIN-DEPENDENT OXIDASES HAVING CANNABINOID SYNTHASE ACTIVITY</title><date>2022-03-10</date><risdate>2022</risdate><abstract>The disclosure relates to a non-natural flavin-dependent oxidase comprising at least one amino acid variation as compared to a wild type flavin-dependent oxidase, wherein the non-natural flavin-dependent oxidase does not comprise a disulfide bond, and wherein the non-natural flavin-dependent oxidase is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid. The disclosure also relates to a nucleic acid, an expression construct, and an engineered cell for making the non-natural flavin-dependent oxidase. Also provided are compositions comprising the non-natural flavin-dependent oxidase; isolated non-natural flavin-dependent oxidase and methods of making the same; cell extracts comprising the non-natural flavin-dependent oxidase; and methods of making cannabinoids.
L'invention concerne une oxydase flavine-dépendante non naturelle comprenant au moins une variation d'acide aminé par comparaison avec une oxydase flavine-dépendante de type sauvage, l'oxydase flavine-dépendante non naturelle ne comprenant pas de liaison disulfure, et l'oxydase flavine-dépendante non naturelle étant capable de cyclisation oxydative d'un composé aromatique prénylé en un cannabinoïde. L'invention concerne également un acide nucléique, une construction d'expression et une cellule modifiée pour préparer l'oxydase flavine-dépendante non naturelle. L'invention concerne également des compositions comprenant l'oxydase flavine-dépendante non naturelle; une oxydase flavine-dépendante non naturelle isolée et leurs procédés de fabrication; des extraits cellulaires comprenant l'oxydase flavine-dépendante non naturelle; et des procédés de fabrication de cannabinoïdes.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng ; fre |
| recordid | cdi_epo_espacenet_CA3190913A1 |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | FLAVIN-DEPENDENT OXIDASES HAVING CANNABINOID SYNTHASE ACTIVITY |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T08%3A55%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=HUDDLESTON,%20JAMISON%20PARKER&rft.date=2022-03-10&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECA3190913A1%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |