HEPES-CONTAINING MEDIUM
The purpose of the present invention is to provide a medium for cell culture that permits cell culture without exhibiting toxicity when a high-concentration HEPES buffer is used, a method for producing said medium, and a cell culture method in which the medium is used. A medium for cell culture cont...
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| creator | OGAWA, SHIMPEI KITAZAWA, MANABU SUZUKI, YU |
| description | The purpose of the present invention is to provide a medium for cell culture that permits cell culture without exhibiting toxicity when a high-concentration HEPES buffer is used, a method for producing said medium, and a cell culture method in which the medium is used. A medium for cell culture containing 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) in a concentration of 30 mM or higher and having an osmotic pressure of 220-340 mOsm/kg, and a cell culture method in which the medium is used, make it possible to improve the cell proliferation rate.
L'objectif de la présente invention est de fournir un milieu de culture cellulaire qui permet une culture cellulaire sans présenter de toxicité lorsqu'un tampon HEPES à haute concentration est utilisé, un procédé de production dudit milieu, et un procédé de culture cellulaire dans lequel le milieu est utilisé. Un milieu de culture cellulaire contenant de l'acide 4- (2-hydroxyéthyl)-1-pipérazineéthanesulfonique (HEPES) dans une concentration de 30 mM ou plus et ayant une pression osmotique de 220-340 mOsm/kg, et un procédé de culture cellulaire dans lequel le milieu est utilisé, permettant d'améliorer le taux de prolifération cellulaire. |
| format | Patent |
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L'objectif de la présente invention est de fournir un milieu de culture cellulaire qui permet une culture cellulaire sans présenter de toxicité lorsqu'un tampon HEPES à haute concentration est utilisé, un procédé de production dudit milieu, et un procédé de culture cellulaire dans lequel le milieu est utilisé. Un milieu de culture cellulaire contenant de l'acide 4- (2-hydroxyéthyl)-1-pipérazineéthanesulfonique (HEPES) dans une concentration de 30 mM ou plus et ayant une pression osmotique de 220-340 mOsm/kg, et un procédé de culture cellulaire dans lequel le milieu est utilisé, permettant d'améliorer le taux de prolifération cellulaire.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210930&DB=EPODOC&CC=CA&NR=3176593A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25569,76552</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210930&DB=EPODOC&CC=CA&NR=3176593A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>OGAWA, SHIMPEI</creatorcontrib><creatorcontrib>KITAZAWA, MANABU</creatorcontrib><creatorcontrib>SUZUKI, YU</creatorcontrib><title>HEPES-CONTAINING MEDIUM</title><description>The purpose of the present invention is to provide a medium for cell culture that permits cell culture without exhibiting toxicity when a high-concentration HEPES buffer is used, a method for producing said medium, and a cell culture method in which the medium is used. A medium for cell culture containing 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) in a concentration of 30 mM or higher and having an osmotic pressure of 220-340 mOsm/kg, and a cell culture method in which the medium is used, make it possible to improve the cell proliferation rate.
L'objectif de la présente invention est de fournir un milieu de culture cellulaire qui permet une culture cellulaire sans présenter de toxicité lorsqu'un tampon HEPES à haute concentration est utilisé, un procédé de production dudit milieu, et un procédé de culture cellulaire dans lequel le milieu est utilisé. Un milieu de culture cellulaire contenant de l'acide 4- (2-hydroxyéthyl)-1-pipérazineéthanesulfonique (HEPES) dans une concentration de 30 mM ou plus et ayant une pression osmotique de 220-340 mOsm/kg, et un procédé de culture cellulaire dans lequel le milieu est utilisé, permettant d'améliorer le taux de prolifération cellulaire.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZBD3cA1wDdZ19vcLcfT08_RzV_B1dfEM9eVhYE1LzClO5YXS3AwKbq4hzh66qQX58anFBYnJqXmpJfHOjsaG5mamlsaOhsZEKAEAluse9Q</recordid><startdate>20210930</startdate><enddate>20210930</enddate><creator>OGAWA, SHIMPEI</creator><creator>KITAZAWA, MANABU</creator><creator>SUZUKI, YU</creator><scope>EVB</scope></search><sort><creationdate>20210930</creationdate><title>HEPES-CONTAINING MEDIUM</title><author>OGAWA, SHIMPEI ; KITAZAWA, MANABU ; SUZUKI, YU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CA3176593A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2021</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>OGAWA, SHIMPEI</creatorcontrib><creatorcontrib>KITAZAWA, MANABU</creatorcontrib><creatorcontrib>SUZUKI, YU</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>OGAWA, SHIMPEI</au><au>KITAZAWA, MANABU</au><au>SUZUKI, YU</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>HEPES-CONTAINING MEDIUM</title><date>2021-09-30</date><risdate>2021</risdate><abstract>The purpose of the present invention is to provide a medium for cell culture that permits cell culture without exhibiting toxicity when a high-concentration HEPES buffer is used, a method for producing said medium, and a cell culture method in which the medium is used. A medium for cell culture containing 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) in a concentration of 30 mM or higher and having an osmotic pressure of 220-340 mOsm/kg, and a cell culture method in which the medium is used, make it possible to improve the cell proliferation rate.
L'objectif de la présente invention est de fournir un milieu de culture cellulaire qui permet une culture cellulaire sans présenter de toxicité lorsqu'un tampon HEPES à haute concentration est utilisé, un procédé de production dudit milieu, et un procédé de culture cellulaire dans lequel le milieu est utilisé. Un milieu de culture cellulaire contenant de l'acide 4- (2-hydroxyéthyl)-1-pipérazineéthanesulfonique (HEPES) dans une concentration de 30 mM ou plus et ayant une pression osmotique de 220-340 mOsm/kg, et un procédé de culture cellulaire dans lequel le milieu est utilisé, permettant d'améliorer le taux de prolifération cellulaire.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | HEPES-CONTAINING MEDIUM |
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