CANNABINOID SYNTHASE VARIANTS AND METHODS FOR THEIR USE
The invention relates to a non-natural cannabinoid synthase comprising at least one amino acid variation as compared to a wild type cannabinoid synthase THCAS, comprising three alpha helices (?A, ?B and ?C) where a disulfide bond is not formed between alpha helix ?A and alpha helix ?C, wherein the n...
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| creator | HUDDLESTON, JAMISON PARKER ZHANG, DEQIANG GRIFFIN, BENJAMIN MATTHEW WARNER, JOSEPH ROY |
| description | The invention relates to a non-natural cannabinoid synthase comprising at least one amino acid variation as compared to a wild type cannabinoid synthase THCAS, comprising three alpha helices (?A, ?B and ?C) where a disulfide bond is not formed between alpha helix ?A and alpha helix ?C, wherein the non-natural cannabinoid synthase catalyzes the oxidative cyclization of CBGA into a cannabinoid. The invention further relates to a non-natural THCAS, a non-natural CBDAS, and a non-natural CBCAS. The invention also relates to a nucleic acid, expression construct, and engineered cell for making the non-natural THCAS, CBDAS, and/or CBCAS. Also provided are compositions comprising the non-natural THCAS, CBDAS, and/or CBCAS; isolated non-natural THCAS, CBDAS, and/or CBCAS enzymes; methods of making the isolated enzymes; cell extracts comprising cannabinoids; and methods of making cannabinoids.
L'invention porte sur une cannabinoïde synthase non naturelle comprenant au moins une variation d'acide aminé en comparaison avec une cannabinoïde synthase THCAS de type sauvage, comprenant trois hélices alpha (?A, ?B and ?C) dans lesquelles un pont disulfure n'étant pas formé entre l'hélice alpha ?A et l'hélice alpha ?C, la cannabinoïde synthase non naturelle catalysant la cyclisation oxydative du CBGA en un cannabinoïde. L'invention porte en outre sur une THCAS non naturelle, une CBDAS non naturelle et une CBCAS non naturelle. L'invention porte également sur un acide nucléique, une construction d'expression et une cellule modifiée pour préparer la THCAS, la CBDAS et/ou la CBCAS non naturelles. L'invention concerne également des compositions comprenant la THCAS, la CBDAS et/ou la CBCAS non naturelles; des enzymes THCAS, CBDAS et/ou CBCAS isolées non naturelles; des procédés de préparation des enzymes isolées; des extraits cellulaires comprenant des cannabinoïdes; et des procédés de préparation de cannabinoïdes. |
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L'invention porte sur une cannabinoïde synthase non naturelle comprenant au moins une variation d'acide aminé en comparaison avec une cannabinoïde synthase THCAS de type sauvage, comprenant trois hélices alpha (?A, ?B and ?C) dans lesquelles un pont disulfure n'étant pas formé entre l'hélice alpha ?A et l'hélice alpha ?C, la cannabinoïde synthase non naturelle catalysant la cyclisation oxydative du CBGA en un cannabinoïde. L'invention porte en outre sur une THCAS non naturelle, une CBDAS non naturelle et une CBCAS non naturelle. L'invention porte également sur un acide nucléique, une construction d'expression et une cellule modifiée pour préparer la THCAS, la CBDAS et/ou la CBCAS non naturelles. L'invention concerne également des compositions comprenant la THCAS, la CBDAS et/ou la CBCAS non naturelles; des enzymes THCAS, CBDAS et/ou CBCAS isolées non naturelles; des procédés de préparation des enzymes isolées; des extraits cellulaires comprenant des cannabinoïdes; et des procédés de préparation de cannabinoïdes.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20211021&DB=EPODOC&CC=CA&NR=3173509A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20211021&DB=EPODOC&CC=CA&NR=3173509A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HUDDLESTON, JAMISON PARKER</creatorcontrib><creatorcontrib>ZHANG, DEQIANG</creatorcontrib><creatorcontrib>GRIFFIN, BENJAMIN MATTHEW</creatorcontrib><creatorcontrib>WARNER, JOSEPH ROY</creatorcontrib><title>CANNABINOID SYNTHASE VARIANTS AND METHODS FOR THEIR USE</title><description>The invention relates to a non-natural cannabinoid synthase comprising at least one amino acid variation as compared to a wild type cannabinoid synthase THCAS, comprising three alpha helices (?A, ?B and ?C) where a disulfide bond is not formed between alpha helix ?A and alpha helix ?C, wherein the non-natural cannabinoid synthase catalyzes the oxidative cyclization of CBGA into a cannabinoid. The invention further relates to a non-natural THCAS, a non-natural CBDAS, and a non-natural CBCAS. The invention also relates to a nucleic acid, expression construct, and engineered cell for making the non-natural THCAS, CBDAS, and/or CBCAS. Also provided are compositions comprising the non-natural THCAS, CBDAS, and/or CBCAS; isolated non-natural THCAS, CBDAS, and/or CBCAS enzymes; methods of making the isolated enzymes; cell extracts comprising cannabinoids; and methods of making cannabinoids.
L'invention porte sur une cannabinoïde synthase non naturelle comprenant au moins une variation d'acide aminé en comparaison avec une cannabinoïde synthase THCAS de type sauvage, comprenant trois hélices alpha (?A, ?B and ?C) dans lesquelles un pont disulfure n'étant pas formé entre l'hélice alpha ?A et l'hélice alpha ?C, la cannabinoïde synthase non naturelle catalysant la cyclisation oxydative du CBGA en un cannabinoïde. L'invention porte en outre sur une THCAS non naturelle, une CBDAS non naturelle et une CBCAS non naturelle. L'invention porte également sur un acide nucléique, une construction d'expression et une cellule modifiée pour préparer la THCAS, la CBDAS et/ou la CBCAS non naturelles. L'invention concerne également des compositions comprenant la THCAS, la CBDAS et/ou la CBCAS non naturelles; des enzymes THCAS, CBDAS et/ou CBCAS isolées non naturelles; des procédés de préparation des enzymes isolées; des extraits cellulaires comprenant des cannabinoïdes; et des procédés de préparation de cannabinoïdes.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDB3dvTzc3Ty9PP3dFEIjvQL8XAMdlUIcwzydPQLCVZw9HNR8HUN8fB3CVZw8w9SCPFw9QxSCA125WFgTUvMKU7lhdLcDApuriHOHrqpBfnxqcUFicmpeakl8c6OxobmxqYGlo6GxkQoAQDpDyeg</recordid><startdate>20211021</startdate><enddate>20211021</enddate><creator>HUDDLESTON, JAMISON PARKER</creator><creator>ZHANG, DEQIANG</creator><creator>GRIFFIN, BENJAMIN MATTHEW</creator><creator>WARNER, JOSEPH ROY</creator><scope>EVB</scope></search><sort><creationdate>20211021</creationdate><title>CANNABINOID SYNTHASE VARIANTS AND METHODS FOR THEIR USE</title><author>HUDDLESTON, JAMISON PARKER ; ZHANG, DEQIANG ; GRIFFIN, BENJAMIN MATTHEW ; WARNER, JOSEPH ROY</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CA3173509A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2021</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HUDDLESTON, JAMISON PARKER</creatorcontrib><creatorcontrib>ZHANG, DEQIANG</creatorcontrib><creatorcontrib>GRIFFIN, BENJAMIN MATTHEW</creatorcontrib><creatorcontrib>WARNER, JOSEPH ROY</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HUDDLESTON, JAMISON PARKER</au><au>ZHANG, DEQIANG</au><au>GRIFFIN, BENJAMIN MATTHEW</au><au>WARNER, JOSEPH ROY</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>CANNABINOID SYNTHASE VARIANTS AND METHODS FOR THEIR USE</title><date>2021-10-21</date><risdate>2021</risdate><abstract>The invention relates to a non-natural cannabinoid synthase comprising at least one amino acid variation as compared to a wild type cannabinoid synthase THCAS, comprising three alpha helices (?A, ?B and ?C) where a disulfide bond is not formed between alpha helix ?A and alpha helix ?C, wherein the non-natural cannabinoid synthase catalyzes the oxidative cyclization of CBGA into a cannabinoid. The invention further relates to a non-natural THCAS, a non-natural CBDAS, and a non-natural CBCAS. The invention also relates to a nucleic acid, expression construct, and engineered cell for making the non-natural THCAS, CBDAS, and/or CBCAS. Also provided are compositions comprising the non-natural THCAS, CBDAS, and/or CBCAS; isolated non-natural THCAS, CBDAS, and/or CBCAS enzymes; methods of making the isolated enzymes; cell extracts comprising cannabinoids; and methods of making cannabinoids.
L'invention porte sur une cannabinoïde synthase non naturelle comprenant au moins une variation d'acide aminé en comparaison avec une cannabinoïde synthase THCAS de type sauvage, comprenant trois hélices alpha (?A, ?B and ?C) dans lesquelles un pont disulfure n'étant pas formé entre l'hélice alpha ?A et l'hélice alpha ?C, la cannabinoïde synthase non naturelle catalysant la cyclisation oxydative du CBGA en un cannabinoïde. L'invention porte en outre sur une THCAS non naturelle, une CBDAS non naturelle et une CBCAS non naturelle. L'invention porte également sur un acide nucléique, une construction d'expression et une cellule modifiée pour préparer la THCAS, la CBDAS et/ou la CBCAS non naturelles. L'invention concerne également des compositions comprenant la THCAS, la CBDAS et/ou la CBCAS non naturelles; des enzymes THCAS, CBDAS et/ou CBCAS isolées non naturelles; des procédés de préparation des enzymes isolées; des extraits cellulaires comprenant des cannabinoïdes; et des procédés de préparation de cannabinoïdes.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | CANNABINOID SYNTHASE VARIANTS AND METHODS FOR THEIR USE |
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