FUSION PROTEINS, RECOMBINANT BACTERIA, AND EXOSPORIUM FRAGMENTS FOR PEST CONTROL AND PLANT HEALTH

The present invention relates to a fusion protein having a targeting sequence, exosporium protein, or exosporium protein fragment that targets the fusion protein to the exosporium of a recombinant Bacillus cereus family member and an enzyme having serine protease activity, wherein the enzyme having...

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Hauptverfasser: CURTIS, DAMIAN, BUGG, KEVIN
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BUGG, KEVIN
description The present invention relates to a fusion protein having a targeting sequence, exosporium protein, or exosporium protein fragment that targets the fusion protein to the exosporium of a recombinant Bacillus cereus family member and an enzyme having serine protease activity, wherein the enzyme having serine protease activity is from Bacillus firmus or is a variant of such enzyme. The present invention also provides a recombinant Bacillus cereus family member that expresses such fusion protein and exosporium fragments derived from such recombinant Bacillus cereus family member. Methods of using such recombinant Bacillus cereus family members or exosporium fragments derived therefrom for nematode control are also provided. La présente invention porte sur une protéine de fusion comportant les éléments suivants : une séquence de ciblage, une protéine d'exospore, ou un fragment de protéine d'exospore qui cible la protéine de fusion vers l'exospore d'un membre recombinant de la famille Bacillus cereus et une enzyme ayant une activité sérine-protéase, l'enzyme ayant une activité sérine-protéase provenant de Bacillus firmus ou étant une variante d'une telle enzyme. La présente invention fournit également un membre recombinant de la famille Bacillus cereus qui exprime cette protéine de fusion et des fragments d'exospore dérivés de ce membre recombinant de la famille Bacillus cereus . L'invention porte également sur des procédés d'utilisation de ces membres recombinants de la famille Bacillus cereus ou de fragments d'exospore dérivés de ceux-ci pour lutter contre les nématodes.
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subjects AGRICULTURE
ANIMAL HUSBANDRY
BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
CHEMISTRY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PEST REPELLANTS OR ATTRACTANTS
PLANT GROWTH REGULATORS
PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF
TRAPPING
title FUSION PROTEINS, RECOMBINANT BACTERIA, AND EXOSPORIUM FRAGMENTS FOR PEST CONTROL AND PLANT HEALTH
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