GLYCOSYLATED LYSOSOMAL PROTEINS, METHOD OF PRODUCTION AND USES
The present invention relates to a lysosomal protein composition comprising a plurality of lysosomal proteins that are potentially diversely glycosylated according to a glycosylation pattern, wherein said glycosylation pattern has at least 45 % paucimannosidic N-glycans; a method of manufacturing th...
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| creator | DABROWSKA-SCHLEPP, PAULINA NIEDERKRUGER, HOLGER BENJAMIN, FODE BUSCH, ANDREAS SCHAAF, ANDREAS |
| description | The present invention relates to a lysosomal protein composition comprising a plurality of lysosomal proteins that are potentially diversely glycosylated according to a glycosylation pattern, wherein said glycosylation pattern has at least 45 % paucimannosidic N-glycans; a method of manufacturing the lysosomal protein composition in a bryophyte plant or cell, and medical and non-medical uses of the lysosomal protein composition. E.g. the lysosomal protein can be a-Galactosidase for the treatment of Fabry Disease or ß-Glucoceramidase for the treatment of Gaucher 's Disease. The unique glycosylation results in improved therapeutic efficacy - surprisingly even without mannose- 6-phosphate that is common for CHO cell produced lysosomal proteins.
La présente invention concerne une composition de protéines lysosomales comprenant une pluralité de protéines lysosomales qui sont potentiellement glycosylées de diverses façons selon un motif de glycosylation, ledit motif de glycosylation ayant au moins 45 % de paucimannosidique N-glycanes ; un procédé de fabrication de la composition de protéines lysosomales dans une plante ou une cellule bryophyte, et des applications médicales et non médicales de la composition de protéines lysosomales. Par exemple la protéine lysosomale peut être une a-galactosidase pour le traitement de la maladie de Fabry ou une ß-glucocéramidase pour le traitement de la maladie de Gaucher. La glycosylation unique permet d'obtenir une meilleure efficacité thérapeutique - étonnamment même sans mannose-6-phosphate qui est courant pour les protéines lysosomales produites par les cellules CHO. |
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La présente invention concerne une composition de protéines lysosomales comprenant une pluralité de protéines lysosomales qui sont potentiellement glycosylées de diverses façons selon un motif de glycosylation, ledit motif de glycosylation ayant au moins 45 % de paucimannosidique N-glycanes ; un procédé de fabrication de la composition de protéines lysosomales dans une plante ou une cellule bryophyte, et des applications médicales et non médicales de la composition de protéines lysosomales. Par exemple la protéine lysosomale peut être une a-galactosidase pour le traitement de la maladie de Fabry ou une ß-glucocéramidase pour le traitement de la maladie de Gaucher. La glycosylation unique permet d'obtenir une meilleure efficacité thérapeutique - étonnamment même sans mannose-6-phosphate qui est courant pour les protéines lysosomales produites par les cellules CHO.</description><language>eng ; fre</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160922&DB=EPODOC&CC=CA&NR=2974050A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160922&DB=EPODOC&CC=CA&NR=2974050A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>DABROWSKA-SCHLEPP, PAULINA</creatorcontrib><creatorcontrib>NIEDERKRUGER, HOLGER</creatorcontrib><creatorcontrib>BENJAMIN, FODE</creatorcontrib><creatorcontrib>BUSCH, ANDREAS</creatorcontrib><creatorcontrib>SCHAAF, ANDREAS</creatorcontrib><title>GLYCOSYLATED LYSOSOMAL PROTEINS, METHOD OF PRODUCTION AND USES</title><description>The present invention relates to a lysosomal protein composition comprising a plurality of lysosomal proteins that are potentially diversely glycosylated according to a glycosylation pattern, wherein said glycosylation pattern has at least 45 % paucimannosidic N-glycans; a method of manufacturing the lysosomal protein composition in a bryophyte plant or cell, and medical and non-medical uses of the lysosomal protein composition. E.g. the lysosomal protein can be a-Galactosidase for the treatment of Fabry Disease or ß-Glucoceramidase for the treatment of Gaucher 's Disease. The unique glycosylation results in improved therapeutic efficacy - surprisingly even without mannose- 6-phosphate that is common for CHO cell produced lysosomal proteins.
La présente invention concerne une composition de protéines lysosomales comprenant une pluralité de protéines lysosomales qui sont potentiellement glycosylées de diverses façons selon un motif de glycosylation, ledit motif de glycosylation ayant au moins 45 % de paucimannosidique N-glycanes ; un procédé de fabrication de la composition de protéines lysosomales dans une plante ou une cellule bryophyte, et des applications médicales et non médicales de la composition de protéines lysosomales. Par exemple la protéine lysosomale peut être une a-galactosidase pour le traitement de la maladie de Fabry ou une ß-glucocéramidase pour le traitement de la maladie de Gaucher. 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E.g. the lysosomal protein can be a-Galactosidase for the treatment of Fabry Disease or ß-Glucoceramidase for the treatment of Gaucher 's Disease. The unique glycosylation results in improved therapeutic efficacy - surprisingly even without mannose- 6-phosphate that is common for CHO cell produced lysosomal proteins.
La présente invention concerne une composition de protéines lysosomales comprenant une pluralité de protéines lysosomales qui sont potentiellement glycosylées de diverses façons selon un motif de glycosylation, ledit motif de glycosylation ayant au moins 45 % de paucimannosidique N-glycanes ; un procédé de fabrication de la composition de protéines lysosomales dans une plante ou une cellule bryophyte, et des applications médicales et non médicales de la composition de protéines lysosomales. Par exemple la protéine lysosomale peut être une a-galactosidase pour le traitement de la maladie de Fabry ou une ß-glucocéramidase pour le traitement de la maladie de Gaucher. La glycosylation unique permet d'obtenir une meilleure efficacité thérapeutique - étonnamment même sans mannose-6-phosphate qui est courant pour les protéines lysosomales produites par les cellules CHO.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | GLYCOSYLATED LYSOSOMAL PROTEINS, METHOD OF PRODUCTION AND USES |
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