ECONOMICAL PROCESS FOR THE ISOLATION OF FUNCTIONAL PROTEIN FROM PLANTS
The invention relates to a method for obtaining a protein from a plant material, wherein the method comprises the steps of i) mechanically disrupting the plant cells to obtain a plant juice in the presence of a reducing agent, ii) treating the plant juice to cause aggregation of chloroplast membrane...
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| creator | HYLKEMA, NIENKE BUSSMANN, PAULUS JOSEPHUS THEODORUS DE JONG, GOVARDUS ADRIANUS HUBERTUS GEERDINK, PETER |
| description | The invention relates to a method for obtaining a protein from a plant material, wherein the method comprises the steps of i) mechanically disrupting the plant cells to obtain a plant juice in the presence of a reducing agent, ii) treating the plant juice to cause aggregation of chloroplast membranes, iii) removing the aggregated chloroplast membranes by precipitation and/or microfiltration, iv) subjecting the plant juice to ultrafiltration, and v) subjecting the soluble plant protein concentrate to hydrophobic column adsorption to remove residual chlorophyll, phenolic compounds and off-odors in a single column passage. The present invention also pertains to an apparatus and system for plant protein isolation based on this method. The isolated proteins can be economically obtained in batch scale and in large scale. Further, the invention is directed to a protein obtained by the method of the invention, a food product comprising thereof, and a use thereof.
L'invention concerne un procédé pour obtenir une protéine à partir d'une matière végétale, le procédé comprenant les étapes consistant à i) perturber mécaniquement les cellules de plante pour obtenir un jus de plante en présence d'un agent réducteur, ii) traiter le jus de plante pour entraîner l'agrégation de membranes de chloroplaste, iii) retirer les membranes de chloroplaste agrégées par précipitation et/ou microfiltration, iv) soumettre le jus de plante à une ultrafiltration, et v) soumettre le concentré de protéine de plante soluble à une adsorption de colonne hydrophobe pour éliminer la chlorophylle résiduelle, les composés phénoliques et les mauvaises odeurs dans un passage de colonne unique. La présente invention concerne également un appareil et un système pour l'isolement de protéine de plante sur la base de ce procédé. Les protéines isolées peuvent être obtenues de façon économique par lots et à grande échelle. En outre, l'invention concerne une protéine obtenue par le procédé de l'invention, un produit alimentaire la comprenant et son utilisation. |
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L'invention concerne un procédé pour obtenir une protéine à partir d'une matière végétale, le procédé comprenant les étapes consistant à i) perturber mécaniquement les cellules de plante pour obtenir un jus de plante en présence d'un agent réducteur, ii) traiter le jus de plante pour entraîner l'agrégation de membranes de chloroplaste, iii) retirer les membranes de chloroplaste agrégées par précipitation et/ou microfiltration, iv) soumettre le jus de plante à une ultrafiltration, et v) soumettre le concentré de protéine de plante soluble à une adsorption de colonne hydrophobe pour éliminer la chlorophylle résiduelle, les composés phénoliques et les mauvaises odeurs dans un passage de colonne unique. La présente invention concerne également un appareil et un système pour l'isolement de protéine de plante sur la base de ce procédé. Les protéines isolées peuvent être obtenues de façon économique par lots et à grande échelle. En outre, l'invention concerne une protéine obtenue par le procédé de l'invention, un produit alimentaire la comprenant et son utilisation.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FOODS OR FOODSTUFFS ; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J ; HUMAN NECESSITIES ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS ; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; PROTEIN COMPOSITIONS FOR FOODSTUFFS ; SPIRITS ; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT ; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES ; VINEGAR ; WINE ; WORKING-UP PROTEINS FOR FOODSTUFFS</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200818&DB=EPODOC&CC=CA&NR=2896227C$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200818&DB=EPODOC&CC=CA&NR=2896227C$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HYLKEMA, NIENKE</creatorcontrib><creatorcontrib>BUSSMANN, PAULUS JOSEPHUS THEODORUS</creatorcontrib><creatorcontrib>DE JONG, GOVARDUS ADRIANUS HUBERTUS</creatorcontrib><creatorcontrib>GEERDINK, PETER</creatorcontrib><title>ECONOMICAL PROCESS FOR THE ISOLATION OF FUNCTIONAL PROTEIN FROM PLANTS</title><description>The invention relates to a method for obtaining a protein from a plant material, wherein the method comprises the steps of i) mechanically disrupting the plant cells to obtain a plant juice in the presence of a reducing agent, ii) treating the plant juice to cause aggregation of chloroplast membranes, iii) removing the aggregated chloroplast membranes by precipitation and/or microfiltration, iv) subjecting the plant juice to ultrafiltration, and v) subjecting the soluble plant protein concentrate to hydrophobic column adsorption to remove residual chlorophyll, phenolic compounds and off-odors in a single column passage. The present invention also pertains to an apparatus and system for plant protein isolation based on this method. The isolated proteins can be economically obtained in batch scale and in large scale. Further, the invention is directed to a protein obtained by the method of the invention, a food product comprising thereof, and a use thereof.
L'invention concerne un procédé pour obtenir une protéine à partir d'une matière végétale, le procédé comprenant les étapes consistant à i) perturber mécaniquement les cellules de plante pour obtenir un jus de plante en présence d'un agent réducteur, ii) traiter le jus de plante pour entraîner l'agrégation de membranes de chloroplaste, iii) retirer les membranes de chloroplaste agrégées par précipitation et/ou microfiltration, iv) soumettre le jus de plante à une ultrafiltration, et v) soumettre le concentré de protéine de plante soluble à une adsorption de colonne hydrophobe pour éliminer la chlorophylle résiduelle, les composés phénoliques et les mauvaises odeurs dans un passage de colonne unique. La présente invention concerne également un appareil et un système pour l'isolement de protéine de plante sur la base de ce procédé. Les protéines isolées peuvent être obtenues de façon économique par lots et à grande échelle. En outre, l'invention concerne une protéine obtenue par le procédé de l'invention, un produit alimentaire la comprenant et son utilisation.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FOODS OR FOODSTUFFS</subject><subject>FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J</subject><subject>HUMAN NECESSITIES</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS</subject><subject>PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>PROTEIN COMPOSITIONS FOR FOODSTUFFS</subject><subject>SPIRITS</subject><subject>THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT</subject><subject>THEIR TREATMENT, NOT COVERED BY OTHER CLASSES</subject><subject>VINEGAR</subject><subject>WINE</subject><subject>WORKING-UP PROTEINS FOR FOODSTUFFS</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHBzdfb38_f1dHb0UQgI8nd2DQ5WcPMPUgjxcFXwDPb3cQzx9PdT8HdTcAv1cwaxIepCXD39FNyC_H0VAnwc_UKCeRhY0xJzilN5oTQ3g7yba4izh25qQX58anFBYnJqXmpJvLOjkYWlmZGRubMxYRUAEbIrnQ</recordid><startdate>20200818</startdate><enddate>20200818</enddate><creator>HYLKEMA, NIENKE</creator><creator>BUSSMANN, PAULUS JOSEPHUS THEODORUS</creator><creator>DE JONG, GOVARDUS ADRIANUS HUBERTUS</creator><creator>GEERDINK, PETER</creator><scope>EVB</scope></search><sort><creationdate>20200818</creationdate><title>ECONOMICAL PROCESS FOR THE ISOLATION OF FUNCTIONAL PROTEIN FROM PLANTS</title><author>HYLKEMA, NIENKE ; BUSSMANN, PAULUS JOSEPHUS THEODORUS ; DE JONG, GOVARDUS ADRIANUS HUBERTUS ; GEERDINK, PETER</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CA2896227C3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FOODS OR FOODSTUFFS</topic><topic>FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J</topic><topic>HUMAN NECESSITIES</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS</topic><topic>PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>PROTEIN COMPOSITIONS FOR FOODSTUFFS</topic><topic>SPIRITS</topic><topic>THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT</topic><topic>THEIR TREATMENT, NOT COVERED BY OTHER CLASSES</topic><topic>VINEGAR</topic><topic>WINE</topic><topic>WORKING-UP PROTEINS FOR FOODSTUFFS</topic><toplevel>online_resources</toplevel><creatorcontrib>HYLKEMA, NIENKE</creatorcontrib><creatorcontrib>BUSSMANN, PAULUS JOSEPHUS THEODORUS</creatorcontrib><creatorcontrib>DE JONG, GOVARDUS ADRIANUS HUBERTUS</creatorcontrib><creatorcontrib>GEERDINK, PETER</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HYLKEMA, NIENKE</au><au>BUSSMANN, PAULUS JOSEPHUS THEODORUS</au><au>DE JONG, GOVARDUS ADRIANUS HUBERTUS</au><au>GEERDINK, PETER</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>ECONOMICAL PROCESS FOR THE ISOLATION OF FUNCTIONAL PROTEIN FROM PLANTS</title><date>2020-08-18</date><risdate>2020</risdate><abstract>The invention relates to a method for obtaining a protein from a plant material, wherein the method comprises the steps of i) mechanically disrupting the plant cells to obtain a plant juice in the presence of a reducing agent, ii) treating the plant juice to cause aggregation of chloroplast membranes, iii) removing the aggregated chloroplast membranes by precipitation and/or microfiltration, iv) subjecting the plant juice to ultrafiltration, and v) subjecting the soluble plant protein concentrate to hydrophobic column adsorption to remove residual chlorophyll, phenolic compounds and off-odors in a single column passage. The present invention also pertains to an apparatus and system for plant protein isolation based on this method. The isolated proteins can be economically obtained in batch scale and in large scale. Further, the invention is directed to a protein obtained by the method of the invention, a food product comprising thereof, and a use thereof.
L'invention concerne un procédé pour obtenir une protéine à partir d'une matière végétale, le procédé comprenant les étapes consistant à i) perturber mécaniquement les cellules de plante pour obtenir un jus de plante en présence d'un agent réducteur, ii) traiter le jus de plante pour entraîner l'agrégation de membranes de chloroplaste, iii) retirer les membranes de chloroplaste agrégées par précipitation et/ou microfiltration, iv) soumettre le jus de plante à une ultrafiltration, et v) soumettre le concentré de protéine de plante soluble à une adsorption de colonne hydrophobe pour éliminer la chlorophylle résiduelle, les composés phénoliques et les mauvaises odeurs dans un passage de colonne unique. La présente invention concerne également un appareil et un système pour l'isolement de protéine de plante sur la base de ce procédé. Les protéines isolées peuvent être obtenues de façon économique par lots et à grande échelle. En outre, l'invention concerne une protéine obtenue par le procédé de l'invention, un produit alimentaire la comprenant et son utilisation.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS PROTEIN COMPOSITIONS FOR FOODSTUFFS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE WORKING-UP PROTEINS FOR FOODSTUFFS |
| title | ECONOMICAL PROCESS FOR THE ISOLATION OF FUNCTIONAL PROTEIN FROM PLANTS |
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