METHODS FOR RAPID MULTIPLEXED AMPLIFICATION OF TARGET NUCLEIC ACIDS
Methods are described for simultaneously amplifying loci in a nucleic acid solution, which include providing samples contained in a plurality of reaction chambers located in a biochip, providing a temperature control element (TCE) for measuring and controlling temperature in the samples and simultan...
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| creator | GIESE, HEIDI S KELLOGG, GREGORY J WRIGHT, JOHN A SELDEN, RICHARD F TAN, EUGENE LAM, HEUNG C |
| description | Methods are described for simultaneously amplifying loci in a nucleic acid solution, which include providing samples contained in a plurality of reaction chambers located in a biochip, providing a temperature control element (TCE) for measuring and controlling temperature in the samples and simultaneously amplifying said loci by using the TCE to sequentially thermally cycle the temperature of the sample in each reaction chamber between a denaturing state, an annealing state, and an extension state for a predetermined number of cycles at heating and cooling rates.
Linvention décrit des procédés pour amplifier simultanément des locus dans une solution dacides nucléiques, lesquels comprennent la fourniture déchantillons contenus dans une pluralité de chambres de réaction situées dans une biopuce, offrant un élément de régulation de la température (TCE) pour mesurer et contrôler la température dans les échantillons et amplifiant simultanément lesdits locus en utilisant le TCE pour cycler de manière séquentielle et thermale la température de léchantillon dans chaque chambre de réaction entre un état de dénaturation, un état de renaturation et un état dextension pour un nombre prédéterminé de cycles à des taux de chauffage et de refroidissement. |
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Linvention décrit des procédés pour amplifier simultanément des locus dans une solution dacides nucléiques, lesquels comprennent la fourniture déchantillons contenus dans une pluralité de chambres de réaction situées dans une biopuce, offrant un élément de régulation de la température (TCE) pour mesurer et contrôler la température dans les échantillons et amplifiant simultanément lesdits locus en utilisant le TCE pour cycler de manière séquentielle et thermale la température de léchantillon dans chaque chambre de réaction entre un état de dénaturation, un état de renaturation et un état dextension pour un nombre prédéterminé de cycles à des taux de chauffage et de refroidissement.</description><language>eng ; fre</language><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2018</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20180109&DB=EPODOC&CC=CA&NR=2894945C$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76294</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20180109&DB=EPODOC&CC=CA&NR=2894945C$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>GIESE, HEIDI S</creatorcontrib><creatorcontrib>KELLOGG, GREGORY J</creatorcontrib><creatorcontrib>WRIGHT, JOHN A</creatorcontrib><creatorcontrib>SELDEN, RICHARD F</creatorcontrib><creatorcontrib>TAN, EUGENE</creatorcontrib><creatorcontrib>LAM, HEUNG C</creatorcontrib><title>METHODS FOR RAPID MULTIPLEXED AMPLIFICATION OF TARGET NUCLEIC ACIDS</title><description>Methods are described for simultaneously amplifying loci in a nucleic acid solution, which include providing samples contained in a plurality of reaction chambers located in a biochip, providing a temperature control element (TCE) for measuring and controlling temperature in the samples and simultaneously amplifying said loci by using the TCE to sequentially thermally cycle the temperature of the sample in each reaction chamber between a denaturing state, an annealing state, and an extension state for a predetermined number of cycles at heating and cooling rates.
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Linvention décrit des procédés pour amplifier simultanément des locus dans une solution dacides nucléiques, lesquels comprennent la fourniture déchantillons contenus dans une pluralité de chambres de réaction situées dans une biopuce, offrant un élément de régulation de la température (TCE) pour mesurer et contrôler la température dans les échantillons et amplifiant simultanément lesdits locus en utilisant le TCE pour cycler de manière séquentielle et thermale la température de léchantillon dans chaque chambre de réaction entre un état de dénaturation, un état de renaturation et un état dextension pour un nombre prédéterminé de cycles à des taux de chauffage et de refroidissement.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING SPIRITS VINEGAR WINE |
| title | METHODS FOR RAPID MULTIPLEXED AMPLIFICATION OF TARGET NUCLEIC ACIDS |
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