METHOD FOR PRODUCING, ISOLATING AND PURIFYING RECOMBINANT HUMAN ANTITRYPTASE (OSRAAT) FROM RICE SEEDS

Provided in the present invention are a codon optimized OsrAAT inherited rice gene, related vector and method for preparing OsrAAT transgenic rice seeds and method for isolating and purifying OsrAAT. OsrAAT transgenic rice seeds are prepared by the specific expression of the expression vector of Osr...

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Bibliographische Detailangaben
Hauptverfasser:	SHI, QIANNI, YANG, DAICHANG, ZHANG, LIPING
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided in the present invention are a codon optimized OsrAAT inherited rice gene, related vector and method for preparing OsrAAT transgenic rice seeds and method for isolating and purifying OsrAAT. OsrAAT transgenic rice seeds are prepared by the specific expression of the expression vector of OsrAAT through the rice albuminous cell, from which OsrAAT is isolated and purified, the obtained OsrAAT having an HPLC purity of 97% and a yield of up to 18.89 ± 3.19%, thus corresponding to being able to produce 0.366 g of OsrAAT per kilogram of unrefined rice.