FUSION PROTEINS TO FACILITATE SELECTION OF CELLS INFECTED WITH SPECIFIC IMMUNOGLOBULIN GENE RECOMBINANT VACCINIA VIRUS
The present invention relates to a high efficiency method of expressing immunoglobulin molecules in eukaryotic cells. The invention is further drawn to a method of producing immunoglobulin heavy and light chain libraries, particularly using the trimolecular recombination method, for expression in eu...
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| creator | PARIS, MARK PANDINA, TRACY CROY, LESLIE A MOKSA, ANGELICA SMITH, ERNEST S KIRK, RENEE ZAUDERER, MAURICE |
| description | The present invention relates to a high efficiency method of expressing immunoglobulin molecules in eukaryotic cells. The invention is further drawn to a method of producing immunoglobulin heavy and light chain libraries, particularly using the trimolecular recombination method, for expression in eukaryotic cells. The invention further provides methods of selecting and screening for antigen-specific immunoglobulin molecules, and antigen-specific fragments thereof. The invention also provides kits for producing, screening and selecting antigen-specific immunoglobulin molecules. Finally, the invention provides immunoglobulin molecules, and antigen-specific fragments thereof, produced by the methods provided herein.
La présente invention concerne une méthode très efficace d'expression de molécules d'immunoglobulines dans des cellules eucaryotes. L'invention concerne, en outre, un procédé de production de banques de chaînes lourdes et légères d'immunoglobulines, faisant notamment appel au procédé de recombinaison trimoléculaire, en vue de leur expression dans des cellules eucaryotes. L'invention concerne, de plus, des procédés de sélection et de criblage de molécules d'immunoglobulines spécifiques d'un antigène et des fragments spécifiques d'un antigène de celles-ci. L'invention concerne également des nécessaires de production, de criblage et de sélection de molécules d'immunoglobulines spécifiques d'un antigène. Enfin, l'invention concerne des molécules d'immunoglobulines et des fragments spécifiques d'un antigène de celles-ci, produits par les procédés de l'invention. |
| format | Patent |
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La présente invention concerne une méthode très efficace d'expression de molécules d'immunoglobulines dans des cellules eucaryotes. L'invention concerne, en outre, un procédé de production de banques de chaînes lourdes et légères d'immunoglobulines, faisant notamment appel au procédé de recombinaison trimoléculaire, en vue de leur expression dans des cellules eucaryotes. L'invention concerne, de plus, des procédés de sélection et de criblage de molécules d'immunoglobulines spécifiques d'un antigène et des fragments spécifiques d'un antigène de celles-ci. L'invention concerne également des nécessaires de production, de criblage et de sélection de molécules d'immunoglobulines spécifiques d'un antigène. Enfin, l'invention concerne des molécules d'immunoglobulines et des fragments spécifiques d'un antigène de celles-ci, produits par les procédés de l'invention.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMBINATORIAL CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210420&DB=EPODOC&CC=CA&NR=2871597C$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210420&DB=EPODOC&CC=CA&NR=2871597C$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>PARIS, MARK</creatorcontrib><creatorcontrib>PANDINA, TRACY</creatorcontrib><creatorcontrib>CROY, LESLIE A</creatorcontrib><creatorcontrib>MOKSA, ANGELICA</creatorcontrib><creatorcontrib>SMITH, ERNEST S</creatorcontrib><creatorcontrib>KIRK, RENEE</creatorcontrib><creatorcontrib>ZAUDERER, MAURICE</creatorcontrib><title>FUSION PROTEINS TO FACILITATE SELECTION OF CELLS INFECTED WITH SPECIFIC IMMUNOGLOBULIN GENE RECOMBINANT VACCINIA VIRUS</title><description>The present invention relates to a high efficiency method of expressing immunoglobulin molecules in eukaryotic cells. The invention is further drawn to a method of producing immunoglobulin heavy and light chain libraries, particularly using the trimolecular recombination method, for expression in eukaryotic cells. The invention further provides methods of selecting and screening for antigen-specific immunoglobulin molecules, and antigen-specific fragments thereof. The invention also provides kits for producing, screening and selecting antigen-specific immunoglobulin molecules. Finally, the invention provides immunoglobulin molecules, and antigen-specific fragments thereof, produced by the methods provided herein.
La présente invention concerne une méthode très efficace d'expression de molécules d'immunoglobulines dans des cellules eucaryotes. L'invention concerne, en outre, un procédé de production de banques de chaînes lourdes et légères d'immunoglobulines, faisant notamment appel au procédé de recombinaison trimoléculaire, en vue de leur expression dans des cellules eucaryotes. L'invention concerne, de plus, des procédés de sélection et de criblage de molécules d'immunoglobulines spécifiques d'un antigène et des fragments spécifiques d'un antigène de celles-ci. L'invention concerne également des nécessaires de production, de criblage et de sélection de molécules d'immunoglobulines spécifiques d'un antigène. 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The invention is further drawn to a method of producing immunoglobulin heavy and light chain libraries, particularly using the trimolecular recombination method, for expression in eukaryotic cells. The invention further provides methods of selecting and screening for antigen-specific immunoglobulin molecules, and antigen-specific fragments thereof. The invention also provides kits for producing, screening and selecting antigen-specific immunoglobulin molecules. Finally, the invention provides immunoglobulin molecules, and antigen-specific fragments thereof, produced by the methods provided herein.
La présente invention concerne une méthode très efficace d'expression de molécules d'immunoglobulines dans des cellules eucaryotes. L'invention concerne, en outre, un procédé de production de banques de chaînes lourdes et légères d'immunoglobulines, faisant notamment appel au procédé de recombinaison trimoléculaire, en vue de leur expression dans des cellules eucaryotes. L'invention concerne, de plus, des procédés de sélection et de criblage de molécules d'immunoglobulines spécifiques d'un antigène et des fragments spécifiques d'un antigène de celles-ci. L'invention concerne également des nécessaires de production, de criblage et de sélection de molécules d'immunoglobulines spécifiques d'un antigène. Enfin, l'invention concerne des molécules d'immunoglobulines et des fragments spécifiques d'un antigène de celles-ci, produits par les procédés de l'invention.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | FUSION PROTEINS TO FACILITATE SELECTION OF CELLS INFECTED WITH SPECIFIC IMMUNOGLOBULIN GENE RECOMBINANT VACCINIA VIRUS |
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