CLONING, EXPRESSION AND USE OF ACID PHOSPHOLIPASES
The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID No: 1, b) DNA sequences that comp...
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| creator | NGUYEN, QUOC KHANH MARSCHNER, VOLKER PALADINO, SILVIA SCHWARZ, TATIANA TITZE, KORNELIA LORENZ, PATRICK |
| description | The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID No: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for with the restriction map by the pPL3940-Topo2.5 plasmid according to figure 7 and that are stored under number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) in the basis of the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: a or a sequence derived therefrom, which can be obtained by substituting, adding, or deleting one or more amino acids, c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide for which a nucleic acid sequence codes, which is hybridized under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acids, f) allelic variants for amino acid sequences a) to e).
L'invention concerne une séquence ADN codant, sensiblement sans activité lipase, un polypeptide ayant une activité phospholipase, et se caractérise en ce que la séquence ADN est sélectionnée dans la liste suivantes: a) séquences ADN qui comprennent une séquence nucléotide conforme à SEQ ID NO: 1, b) séquences ADN qui comprennent la séquence codante conforme à SEQ ID NO: 1, c) séquences ADN qui codent la séquence protéine conforme |
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| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CA2778668A1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CA2778668A1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CA2778668A13</originalsourceid><addsrcrecordid>eNrjZDBy9vH38_Rz11FwjQgIcg0O9vT3U3D0c1EIDXZV8HdTcHT2dFEI8PAPBmIfzwDHYNdgHgbWtMSc4lReKM3NoODmGuLsoZtakB-fWlyQmJyal1oS7-xoZG5uYWZm4WhoTIQSACEJJlU</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>CLONING, EXPRESSION AND USE OF ACID PHOSPHOLIPASES</title><source>esp@cenet</source><creator>NGUYEN, QUOC KHANH ; MARSCHNER, VOLKER ; PALADINO, SILVIA ; SCHWARZ, TATIANA ; TITZE, KORNELIA ; LORENZ, PATRICK</creator><creatorcontrib>NGUYEN, QUOC KHANH ; MARSCHNER, VOLKER ; PALADINO, SILVIA ; SCHWARZ, TATIANA ; TITZE, KORNELIA ; LORENZ, PATRICK</creatorcontrib><description>The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID No: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for with the restriction map by the pPL3940-Topo2.5 plasmid according to figure 7 and that are stored under number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) in the basis of the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: a or a sequence derived therefrom, which can be obtained by substituting, adding, or deleting one or more amino acids, c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide for which a nucleic acid sequence codes, which is hybridized under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acids, f) allelic variants for amino acid sequences a) to e).
L'invention concerne une séquence ADN codant, sensiblement sans activité lipase, un polypeptide ayant une activité phospholipase, et se caractérise en ce que la séquence ADN est sélectionnée dans la liste suivantes: a) séquences ADN qui comprennent une séquence nucléotide conforme à SEQ ID NO: 1, b) séquences ADN qui comprennent la séquence codante conforme à SEQ ID NO: 1, c) séquences ADN qui codent la séquence protéine conforme à SEQ ID NO: 2 d) séquences ADN qui sont codées par le plasmide pPL3940-Topo2.5, avec la carte de restriction conforme à la figure 7, et déposées sous le numéro de dépôt DSM 22741, e) séquences ADN qui, dans des conditions stringentes, sont hybridées avec l'une des séquences ADN, conformément à a), b), c) ou d), f) séquences ADN qui sont apparentées, par suite de la dégénération du code génétique, avec les séquences ADN conformément à a), b), c), d) ou e), et g) brins complémentaires aux séquences, conformément à a) à f), la séquence ADN étant dérivée, de préférence, de l'Aspergillus et, en particulier, de l'Aspergillus fumigatus; et un polypeptide ayant une activité phospholipase, sensiblement sans activité lipase, sélectionné dans la liste suivante: a) un polypeptide qui est codé comme précédemment défini, par la partie codante d'une séquence ADN, b) un polypeptide ayant la séquence conforme à SEQ ID NO: 2 ou une séquence qui en dérive, qui est obtenue par substitution, addition, délétion d'un ou de plusieurs aminoacides, c) un polypeptide ayant une séquence qui présente au moins 83% d'identité par rapport aux aminoacides 1 à 299 de SEQ ID NO: 2, d) un polypeptide qui est codé par une séquence d'acide nucléique qui est hybridée, dans des conditions stringentes, avec (i) les nucléotides 55 à 1106 de SEQ ID NO: 1, (ii) la séquence ADNc contenue dans les nucléotides 55 à 1 106 de SEQ ID NO: 1, (iii) une séquence partielle de (i) ou (ii) d'au moins 100 nucléotides, ou (iv) d'un brin complémentaire de (i), (ii) ou (iii), e) une variante du polypeptide avec SEQ ID NO: 2 comprenant une substitution, une délétion et/ou une insertion d'un ou de plusieurs aminoacides, f) variantes alléliques des séquences aminoacides a) à e).</description><language>eng ; fre</language><subject>ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES ; BEER ; BIOCHEMISTRY ; BLEACHING LEATHER OR FURS ; CANDLES ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; DETERGENTS ; DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS,YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS ; ENZYMOLOGY ; ESSENTIAL OILS ; FATTY ACIDS THEREFROM ; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR ; LAUNDERING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PERFUMES ; PRODUCING (PRESSING, EXTRACTION), REFINING AND PRESERVINGFATS, FATTY SUBSTANCES (e.g. LANOLIN), FATTY OILS AND WAXES,INCLUDING EXTRACTION FROM WASTE MATERIALS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TEXTILES ; TREATMENT OF TEXTILES OR THE LIKE ; VINEGAR ; WINE</subject><creationdate>2011</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20110505&DB=EPODOC&CC=CA&NR=2778668A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76318</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20110505&DB=EPODOC&CC=CA&NR=2778668A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>NGUYEN, QUOC KHANH</creatorcontrib><creatorcontrib>MARSCHNER, VOLKER</creatorcontrib><creatorcontrib>PALADINO, SILVIA</creatorcontrib><creatorcontrib>SCHWARZ, TATIANA</creatorcontrib><creatorcontrib>TITZE, KORNELIA</creatorcontrib><creatorcontrib>LORENZ, PATRICK</creatorcontrib><title>CLONING, EXPRESSION AND USE OF ACID PHOSPHOLIPASES</title><description>The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID No: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for with the restriction map by the pPL3940-Topo2.5 plasmid according to figure 7 and that are stored under number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) in the basis of the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: a or a sequence derived therefrom, which can be obtained by substituting, adding, or deleting one or more amino acids, c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide for which a nucleic acid sequence codes, which is hybridized under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acids, f) allelic variants for amino acid sequences a) to e).
L'invention concerne une séquence ADN codant, sensiblement sans activité lipase, un polypeptide ayant une activité phospholipase, et se caractérise en ce que la séquence ADN est sélectionnée dans la liste suivantes: a) séquences ADN qui comprennent une séquence nucléotide conforme à SEQ ID NO: 1, b) séquences ADN qui comprennent la séquence codante conforme à SEQ ID NO: 1, c) séquences ADN qui codent la séquence protéine conforme à SEQ ID NO: 2 d) séquences ADN qui sont codées par le plasmide pPL3940-Topo2.5, avec la carte de restriction conforme à la figure 7, et déposées sous le numéro de dépôt DSM 22741, e) séquences ADN qui, dans des conditions stringentes, sont hybridées avec l'une des séquences ADN, conformément à a), b), c) ou d), f) séquences ADN qui sont apparentées, par suite de la dégénération du code génétique, avec les séquences ADN conformément à a), b), c), d) ou e), et g) brins complémentaires aux séquences, conformément à a) à f), la séquence ADN étant dérivée, de préférence, de l'Aspergillus et, en particulier, de l'Aspergillus fumigatus; et un polypeptide ayant une activité phospholipase, sensiblement sans activité lipase, sélectionné dans la liste suivante: a) un polypeptide qui est codé comme précédemment défini, par la partie codante d'une séquence ADN, b) un polypeptide ayant la séquence conforme à SEQ ID NO: 2 ou une séquence qui en dérive, qui est obtenue par substitution, addition, délétion d'un ou de plusieurs aminoacides, c) un polypeptide ayant une séquence qui présente au moins 83% d'identité par rapport aux aminoacides 1 à 299 de SEQ ID NO: 2, d) un polypeptide qui est codé par une séquence d'acide nucléique qui est hybridée, dans des conditions stringentes, avec (i) les nucléotides 55 à 1106 de SEQ ID NO: 1, (ii) la séquence ADNc contenue dans les nucléotides 55 à 1 106 de SEQ ID NO: 1, (iii) une séquence partielle de (i) ou (ii) d'au moins 100 nucléotides, ou (iv) d'un brin complémentaire de (i), (ii) ou (iii), e) une variante du polypeptide avec SEQ ID NO: 2 comprenant une substitution, une délétion et/ou une insertion d'un ou de plusieurs aminoacides, f) variantes alléliques des séquences aminoacides a) à e).</description><subject>ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>BLEACHING LEATHER OR FURS</subject><subject>CANDLES</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>DETERGENTS</subject><subject>DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS,YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS</subject><subject>ENZYMOLOGY</subject><subject>ESSENTIAL OILS</subject><subject>FATTY ACIDS THEREFROM</subject><subject>FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR</subject><subject>LAUNDERING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PERFUMES</subject><subject>PRODUCING (PRESSING, EXTRACTION), REFINING AND PRESERVINGFATS, FATTY SUBSTANCES (e.g. LANOLIN), FATTY OILS AND WAXES,INCLUDING EXTRACTION FROM WASTE MATERIALS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TEXTILES</subject><subject>TREATMENT OF TEXTILES OR THE LIKE</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2011</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDBy9vH38_Rz11FwjQgIcg0O9vT3U3D0c1EIDXZV8HdTcHT2dFEI8PAPBmIfzwDHYNdgHgbWtMSc4lReKM3NoODmGuLsoZtakB-fWlyQmJyal1oS7-xoZG5uYWZm4WhoTIQSACEJJlU</recordid><startdate>20110505</startdate><enddate>20110505</enddate><creator>NGUYEN, QUOC KHANH</creator><creator>MARSCHNER, VOLKER</creator><creator>PALADINO, SILVIA</creator><creator>SCHWARZ, TATIANA</creator><creator>TITZE, KORNELIA</creator><creator>LORENZ, PATRICK</creator><scope>EVB</scope></search><sort><creationdate>20110505</creationdate><title>CLONING, EXPRESSION AND USE OF ACID PHOSPHOLIPASES</title><author>NGUYEN, QUOC KHANH ; MARSCHNER, VOLKER ; PALADINO, SILVIA ; SCHWARZ, TATIANA ; TITZE, KORNELIA ; LORENZ, PATRICK</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CA2778668A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2011</creationdate><topic>ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>BLEACHING LEATHER OR FURS</topic><topic>CANDLES</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>DETERGENTS</topic><topic>DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS,YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS</topic><topic>ENZYMOLOGY</topic><topic>ESSENTIAL OILS</topic><topic>FATTY ACIDS THEREFROM</topic><topic>FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR</topic><topic>LAUNDERING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PERFUMES</topic><topic>PRODUCING (PRESSING, EXTRACTION), REFINING AND PRESERVINGFATS, FATTY SUBSTANCES (e.g. LANOLIN), FATTY OILS AND WAXES,INCLUDING EXTRACTION FROM WASTE MATERIALS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TEXTILES</topic><topic>TREATMENT OF TEXTILES OR THE LIKE</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>NGUYEN, QUOC KHANH</creatorcontrib><creatorcontrib>MARSCHNER, VOLKER</creatorcontrib><creatorcontrib>PALADINO, SILVIA</creatorcontrib><creatorcontrib>SCHWARZ, TATIANA</creatorcontrib><creatorcontrib>TITZE, KORNELIA</creatorcontrib><creatorcontrib>LORENZ, PATRICK</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>NGUYEN, QUOC KHANH</au><au>MARSCHNER, VOLKER</au><au>PALADINO, SILVIA</au><au>SCHWARZ, TATIANA</au><au>TITZE, KORNELIA</au><au>LORENZ, PATRICK</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>CLONING, EXPRESSION AND USE OF ACID PHOSPHOLIPASES</title><date>2011-05-05</date><risdate>2011</risdate><abstract>The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID No: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for with the restriction map by the pPL3940-Topo2.5 plasmid according to figure 7 and that are stored under number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) in the basis of the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: a or a sequence derived therefrom, which can be obtained by substituting, adding, or deleting one or more amino acids, c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide for which a nucleic acid sequence codes, which is hybridized under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acids, f) allelic variants for amino acid sequences a) to e).
L'invention concerne une séquence ADN codant, sensiblement sans activité lipase, un polypeptide ayant une activité phospholipase, et se caractérise en ce que la séquence ADN est sélectionnée dans la liste suivantes: a) séquences ADN qui comprennent une séquence nucléotide conforme à SEQ ID NO: 1, b) séquences ADN qui comprennent la séquence codante conforme à SEQ ID NO: 1, c) séquences ADN qui codent la séquence protéine conforme à SEQ ID NO: 2 d) séquences ADN qui sont codées par le plasmide pPL3940-Topo2.5, avec la carte de restriction conforme à la figure 7, et déposées sous le numéro de dépôt DSM 22741, e) séquences ADN qui, dans des conditions stringentes, sont hybridées avec l'une des séquences ADN, conformément à a), b), c) ou d), f) séquences ADN qui sont apparentées, par suite de la dégénération du code génétique, avec les séquences ADN conformément à a), b), c), d) ou e), et g) brins complémentaires aux séquences, conformément à a) à f), la séquence ADN étant dérivée, de préférence, de l'Aspergillus et, en particulier, de l'Aspergillus fumigatus; et un polypeptide ayant une activité phospholipase, sensiblement sans activité lipase, sélectionné dans la liste suivante: a) un polypeptide qui est codé comme précédemment défini, par la partie codante d'une séquence ADN, b) un polypeptide ayant la séquence conforme à SEQ ID NO: 2 ou une séquence qui en dérive, qui est obtenue par substitution, addition, délétion d'un ou de plusieurs aminoacides, c) un polypeptide ayant une séquence qui présente au moins 83% d'identité par rapport aux aminoacides 1 à 299 de SEQ ID NO: 2, d) un polypeptide qui est codé par une séquence d'acide nucléique qui est hybridée, dans des conditions stringentes, avec (i) les nucléotides 55 à 1106 de SEQ ID NO: 1, (ii) la séquence ADNc contenue dans les nucléotides 55 à 1 106 de SEQ ID NO: 1, (iii) une séquence partielle de (i) ou (ii) d'au moins 100 nucléotides, ou (iv) d'un brin complémentaire de (i), (ii) ou (iii), e) une variante du polypeptide avec SEQ ID NO: 2 comprenant une substitution, une délétion et/ou une insertion d'un ou de plusieurs aminoacides, f) variantes alléliques des séquences aminoacides a) à e).</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY BLEACHING LEATHER OR FURS CANDLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DETERGENTS DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS,YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS ENZYMOLOGY ESSENTIAL OILS FATTY ACIDS THEREFROM FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR LAUNDERING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFUMES PRODUCING (PRESSING, EXTRACTION), REFINING AND PRESERVINGFATS, FATTY SUBSTANCES (e.g. LANOLIN), FATTY OILS AND WAXES,INCLUDING EXTRACTION FROM WASTE MATERIALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TEXTILES TREATMENT OF TEXTILES OR THE LIKE VINEGAR WINE |
| title | CLONING, EXPRESSION AND USE OF ACID PHOSPHOLIPASES |
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