METHODS AND SYSTEMS FOR MEASURING MICROBIOLOGICAL CONTENT IN AQUEOUS MEDIA
A process for measuring total microbiological content in an aqueous medium includes adding a fluorescent dye to the aqueous medium, measuring the fluorescent signal in the aqueous medium to obtain a baseline fluorescent signal, releasing intracellular content of the microbiological matter into the a...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | eng ; fre |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | HIRST, PAUL RONALD CAI, HONG XU, RONG BOYETTE, SCOTT MARTELL JIN, YAN LI, JIE YANG, KECHAO WANG, YU JIANG, JUAN |
| description | A process for measuring total microbiological content in an aqueous medium includes adding a fluorescent dye to the aqueous medium, measuring the fluorescent signal in the aqueous medium to obtain a baseline fluorescent signal, releasing intracellular content of the microbiological matter into the aqueous medium by lysing the microbiological matter, measuring the fluorescent signal in the aqueous medium with the released intracellular content of the microbiological matter to obtain a second fluorescent signal, subtracting the baseline signal from the second fluorescent signal to obtain a net fluorescent signal and equating the net fluorescent signal with a microbiological content. Systems for measuring total microbiological content and methods for measuring biofilm and adjusting for background noise are also provided.
L'invention porte sur un procédé de mesure de la teneur microbiologique totale dans un milieu aqueux, qui comprend les opérations consistant à ajouter un colorant fluorescent au milieu aqueux, à mesurer le signal fluorescent dans le milieu aqueux pour obtenir un signal fluorescent de référence, à libérer le contenu intracellulaire de la matière microbiologique dans le milieu aqueux par lyse de la matière microbiologique, à mesurer le signal fluorescent dans le milieu aqueux avec la teneur intracellulaire libérée de la matière microbiologique et pour obtenir un second signal fluorescent, à soustraire le signal de référence à partir du second signal fluorescent pour obtenir un signal fluorescent net et à faire correspondre le signal fluorescent net avec une teneur microbiologique. L'invention porte également sur des systèmes de mesure de la teneur microbiologique totale et des procédés pour mesurer un biofilm et ajuster le bruit de fond. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CA2741449C</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CA2741449C</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CA2741449C3</originalsourceid><addsrcrecordid>eNqFyrEKwjAQANAsDqJ-g_cDDmpAHM_LtT1pctgkg1MpEifRQv1_dHB3esubm7Pn1KiLgMFBvMbEPkKlHXjGmDsJNXihTk-irdZC2AJpSBwSSAC8ZNYcv9kJLs3sPjymsvq5MOuKEzWbMr76Mo3DrTzLuyfcHezW2iPt_48Pw7ksqg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>METHODS AND SYSTEMS FOR MEASURING MICROBIOLOGICAL CONTENT IN AQUEOUS MEDIA</title><source>esp@cenet</source><creator>HIRST, PAUL RONALD ; CAI, HONG ; XU, RONG ; BOYETTE, SCOTT MARTELL ; JIN, YAN ; LI, JIE ; YANG, KECHAO ; WANG, YU ; JIANG, JUAN</creator><creatorcontrib>HIRST, PAUL RONALD ; CAI, HONG ; XU, RONG ; BOYETTE, SCOTT MARTELL ; JIN, YAN ; LI, JIE ; YANG, KECHAO ; WANG, YU ; JIANG, JUAN</creatorcontrib><description>A process for measuring total microbiological content in an aqueous medium includes adding a fluorescent dye to the aqueous medium, measuring the fluorescent signal in the aqueous medium to obtain a baseline fluorescent signal, releasing intracellular content of the microbiological matter into the aqueous medium by lysing the microbiological matter, measuring the fluorescent signal in the aqueous medium with the released intracellular content of the microbiological matter to obtain a second fluorescent signal, subtracting the baseline signal from the second fluorescent signal to obtain a net fluorescent signal and equating the net fluorescent signal with a microbiological content. Systems for measuring total microbiological content and methods for measuring biofilm and adjusting for background noise are also provided.
L'invention porte sur un procédé de mesure de la teneur microbiologique totale dans un milieu aqueux, qui comprend les opérations consistant à ajouter un colorant fluorescent au milieu aqueux, à mesurer le signal fluorescent dans le milieu aqueux pour obtenir un signal fluorescent de référence, à libérer le contenu intracellulaire de la matière microbiologique dans le milieu aqueux par lyse de la matière microbiologique, à mesurer le signal fluorescent dans le milieu aqueux avec la teneur intracellulaire libérée de la matière microbiologique et pour obtenir un second signal fluorescent, à soustraire le signal de référence à partir du second signal fluorescent pour obtenir un signal fluorescent net et à faire correspondre le signal fluorescent net avec une teneur microbiologique. L'invention porte également sur des systèmes de mesure de la teneur microbiologique totale et des procédés pour mesurer un biofilm et ajuster le bruit de fond.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170613&DB=EPODOC&CC=CA&NR=2741449C$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170613&DB=EPODOC&CC=CA&NR=2741449C$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HIRST, PAUL RONALD</creatorcontrib><creatorcontrib>CAI, HONG</creatorcontrib><creatorcontrib>XU, RONG</creatorcontrib><creatorcontrib>BOYETTE, SCOTT MARTELL</creatorcontrib><creatorcontrib>JIN, YAN</creatorcontrib><creatorcontrib>LI, JIE</creatorcontrib><creatorcontrib>YANG, KECHAO</creatorcontrib><creatorcontrib>WANG, YU</creatorcontrib><creatorcontrib>JIANG, JUAN</creatorcontrib><title>METHODS AND SYSTEMS FOR MEASURING MICROBIOLOGICAL CONTENT IN AQUEOUS MEDIA</title><description>A process for measuring total microbiological content in an aqueous medium includes adding a fluorescent dye to the aqueous medium, measuring the fluorescent signal in the aqueous medium to obtain a baseline fluorescent signal, releasing intracellular content of the microbiological matter into the aqueous medium by lysing the microbiological matter, measuring the fluorescent signal in the aqueous medium with the released intracellular content of the microbiological matter to obtain a second fluorescent signal, subtracting the baseline signal from the second fluorescent signal to obtain a net fluorescent signal and equating the net fluorescent signal with a microbiological content. Systems for measuring total microbiological content and methods for measuring biofilm and adjusting for background noise are also provided.
L'invention porte sur un procédé de mesure de la teneur microbiologique totale dans un milieu aqueux, qui comprend les opérations consistant à ajouter un colorant fluorescent au milieu aqueux, à mesurer le signal fluorescent dans le milieu aqueux pour obtenir un signal fluorescent de référence, à libérer le contenu intracellulaire de la matière microbiologique dans le milieu aqueux par lyse de la matière microbiologique, à mesurer le signal fluorescent dans le milieu aqueux avec la teneur intracellulaire libérée de la matière microbiologique et pour obtenir un second signal fluorescent, à soustraire le signal de référence à partir du second signal fluorescent pour obtenir un signal fluorescent net et à faire correspondre le signal fluorescent net avec une teneur microbiologique. L'invention porte également sur des systèmes de mesure de la teneur microbiologique totale et des procédés pour mesurer un biofilm et ajuster le bruit de fond.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqFyrEKwjAQANAsDqJ-g_cDDmpAHM_LtT1pctgkg1MpEifRQv1_dHB3esubm7Pn1KiLgMFBvMbEPkKlHXjGmDsJNXihTk-irdZC2AJpSBwSSAC8ZNYcv9kJLs3sPjymsvq5MOuKEzWbMr76Mo3DrTzLuyfcHezW2iPt_48Pw7ksqg</recordid><startdate>20170613</startdate><enddate>20170613</enddate><creator>HIRST, PAUL RONALD</creator><creator>CAI, HONG</creator><creator>XU, RONG</creator><creator>BOYETTE, SCOTT MARTELL</creator><creator>JIN, YAN</creator><creator>LI, JIE</creator><creator>YANG, KECHAO</creator><creator>WANG, YU</creator><creator>JIANG, JUAN</creator><scope>EVB</scope></search><sort><creationdate>20170613</creationdate><title>METHODS AND SYSTEMS FOR MEASURING MICROBIOLOGICAL CONTENT IN AQUEOUS MEDIA</title><author>HIRST, PAUL RONALD ; CAI, HONG ; XU, RONG ; BOYETTE, SCOTT MARTELL ; JIN, YAN ; LI, JIE ; YANG, KECHAO ; WANG, YU ; JIANG, JUAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CA2741449C3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2017</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HIRST, PAUL RONALD</creatorcontrib><creatorcontrib>CAI, HONG</creatorcontrib><creatorcontrib>XU, RONG</creatorcontrib><creatorcontrib>BOYETTE, SCOTT MARTELL</creatorcontrib><creatorcontrib>JIN, YAN</creatorcontrib><creatorcontrib>LI, JIE</creatorcontrib><creatorcontrib>YANG, KECHAO</creatorcontrib><creatorcontrib>WANG, YU</creatorcontrib><creatorcontrib>JIANG, JUAN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HIRST, PAUL RONALD</au><au>CAI, HONG</au><au>XU, RONG</au><au>BOYETTE, SCOTT MARTELL</au><au>JIN, YAN</au><au>LI, JIE</au><au>YANG, KECHAO</au><au>WANG, YU</au><au>JIANG, JUAN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHODS AND SYSTEMS FOR MEASURING MICROBIOLOGICAL CONTENT IN AQUEOUS MEDIA</title><date>2017-06-13</date><risdate>2017</risdate><abstract>A process for measuring total microbiological content in an aqueous medium includes adding a fluorescent dye to the aqueous medium, measuring the fluorescent signal in the aqueous medium to obtain a baseline fluorescent signal, releasing intracellular content of the microbiological matter into the aqueous medium by lysing the microbiological matter, measuring the fluorescent signal in the aqueous medium with the released intracellular content of the microbiological matter to obtain a second fluorescent signal, subtracting the baseline signal from the second fluorescent signal to obtain a net fluorescent signal and equating the net fluorescent signal with a microbiological content. Systems for measuring total microbiological content and methods for measuring biofilm and adjusting for background noise are also provided.
L'invention porte sur un procédé de mesure de la teneur microbiologique totale dans un milieu aqueux, qui comprend les opérations consistant à ajouter un colorant fluorescent au milieu aqueux, à mesurer le signal fluorescent dans le milieu aqueux pour obtenir un signal fluorescent de référence, à libérer le contenu intracellulaire de la matière microbiologique dans le milieu aqueux par lyse de la matière microbiologique, à mesurer le signal fluorescent dans le milieu aqueux avec la teneur intracellulaire libérée de la matière microbiologique et pour obtenir un second signal fluorescent, à soustraire le signal de référence à partir du second signal fluorescent pour obtenir un signal fluorescent net et à faire correspondre le signal fluorescent net avec une teneur microbiologique. L'invention porte également sur des systèmes de mesure de la teneur microbiologique totale et des procédés pour mesurer un biofilm et ajuster le bruit de fond.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng ; fre |
| recordid | cdi_epo_espacenet_CA2741449C |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE |
| title | METHODS AND SYSTEMS FOR MEASURING MICROBIOLOGICAL CONTENT IN AQUEOUS MEDIA |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T23%3A59%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=HIRST,%20PAUL%20RONALD&rft.date=2017-06-13&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECA2741449C%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |