YEAST PLATFORM CONSTRUCTION AND SCREENING METHODS

The present invention relates to a cell which is suitable for screening a candidate agent as being an inhibitor of the metabolism of tryptophan to NA D+ and/or a modulator of NAD+ levels, which cell comprises functional genes of a pathway enabling the metabolism of tryptophan to NAD+ and wherein the...

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Bibliographische Detailangaben
Hauptverfasser:	VIEIRA, HELENA MARGARIDA MOREIRA DE OLIVEIRA, RODRIGUES, CATIA SANTANA REVERENDO, CALADO, PATRICIA RAMALHETE MENDES DA SILVA, ROCA, CHRISTOPHE FRANCOIS AIME, PINHEIRO, RICARDO FILIPE ANTUNES, DOS SANTOS, ALEXANDRA MARIA BARROS, SAM, JOHANNES, CEREJO, MARTA ISABEL HEITOR, CHATTERJEE, SUKALYAN, SOUSA, JOSE MANUEL BERNARDO
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a cell which is suitable for screening a candidate agent as being an inhibitor of the metabolism of tryptophan to NA D+ and/or a modulator of NAD+ levels, which cell comprises functional genes of a pathway enabling the metabolism of tryptophan to NAD+ and wherein the c ell includes a copy of an exogenous gene of said pathway, from the same or d ifferent species as the cell, which exogenous gene is under the control of a n inducible or constitutive promoter and wherein any endogenous copy of the gene having the same function as the exogenous gene is a non functioning gen e. The present invention also relates to populations of such cells and to me thods of screening candidate agents with such cells.