METHOD FOR PROTEIN ISOLATION IN ANOXIC CONDITIONS

METHOD FOR PROTEIN ISOLATION IN ANOXIC CONDITIONS

The present invention relates to a method for the isolation of proteins that comprise disulfide-bonds in their native conformation. Essentially, a method of the present makes the use of reducing agents such as β-mercaptoethanol or dithiothreitol in protein isolation methods obsolete. A method of the...

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Hauptverfasser: WASSENAAR, ROELAND WILHELMUS, VAN DER MEIJDEN, PETER, VAN DEDEM, GIJSBERT WILLEM KAREL, EPPINK, MICHEL HENDRIKUS MARIA
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CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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creator WASSENAAR, ROELAND WILHELMUS
VAN DER MEIJDEN, PETER
VAN DEDEM, GIJSBERT WILLEM KAREL
EPPINK, MICHEL HENDRIKUS MARIA
description The present invention relates to a method for the isolation of proteins that comprise disulfide-bonds in their native conformation. Essentially, a method of the present makes the use of reducing agents such as β-mercaptoethanol or dithiothreitol in protein isolation methods obsolete. A method of the present invention is particularly suitable for the isolation of precursor proteins such as proinsulin from recombinant cells.
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subjects CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
title METHOD FOR PROTEIN ISOLATION IN ANOXIC CONDITIONS
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